WTAP Overexpression Lysate (Native)

Images

 
Western Blot: WTAP Overexpression Lysate (Adult Normal) [NBL1-17889] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WTAP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WTAP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209632 and based on accession number NM_004906. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Wilms tumor 1 associated protein (WTAP), transcript variant 1, mRNA.
Gene
WTAP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WTAP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WTAP Overexpression Lysate (Native)

  • Female-lethal(2)D homolog
  • hFL(2)D
  • KIAA0105DKFZp686F20131
  • MGC3925
  • PNAS-132
  • pre-mRNA-splicing regulator WTAP
  • putative pre-mRNA splicing regulator female-lethal(2D)
  • Wilms tumor 1 associated protein
  • Wilms tumor 1-associating protein
  • Wilms' tumour 1-associating protein
  • WT1-associated protein

Background

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for WTAP Lysate (NBL1-17889) (0)

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Bioinformatics

Gene Symbol WTAP