WTAP Antibody (OTI12G7) - Azide and BSA Free

Western Blot: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WTAP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiWTAP.(1:2000)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human lymphoma tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-WTAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human lung tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human pancreas tissue using anti-WTAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human prostate tissue using anti-WTAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human tonsil using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,) (1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human bladder tissue using anti-WTAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human endometrium tissue using anti-WTAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human lymph node tissue using anti-WTAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)

Immunohistochemistry: WTAP Antibody (OTI12G7) - Azide and BSA Free [NBP2-74870] - Staining of paraffin-embedded Human thyroid tissue using anti-WTAP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI12G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

WTAP Antibody (OTI12G7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse WTAP Antibody (OTI12G7) - Azide and BSA Free (NBP2-46368) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-106 of human WTAP(NP_004897) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WTAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 44.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for WTAP Antibody (OTI12G7) - Azide and BSA Free

• Female-lethal(2)D homolog
• hFL(2)D
• KIAA0105DKFZp686F20131
• MGC3925
• PNAS-132
• pre-mRNA-splicing regulator WTAP
• putative pre-mRNA splicing regulator female-lethal(2D)
• Wilms tumor 1 associated protein
• Wilms tumor 1-associating protein
• Wilms' tumour 1-associating protein
• WT1-associated protein

Background

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WTAP