Wnt-5a Antibody [Unconjugated]

Western blot shows lysates of mouse brain and lactating mammary tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑5a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Wnt‑5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-5a Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Wnt‑5a peptide
  Gln254-Cys334
  Accession # P22725
• Specificity: Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT5A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-5a Antibody [Unconjugated]

• hWNT5A
• protein Wnt-5a
• wingless-type MMTV integration site family, member 5A
• WNT-5A protein
• Wnt5a
• Wnt-5a

Background

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca²⁺ pathway, and the planar cell polarity (1, 2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

1. Miller, J.R. (2002) Genome Biol. 3:3001.
2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
4. Topol, L. et al. (2003) J. Cell Biol 162:899.
5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-5a Antibody (AF645)(35)

Publications using AF645

• CS Thomson, J Pundavela, MR Perrino, RA Coover, K Choi, KE Chaney, TA Rizvi, DA Largaespad, N Ratner WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth Oncogene, 2021;40(24):4229-4241. 2021 [PMID: 34079083] (Western Blot, Mouse)
• AC van Vliet, J Lee, M van der Po, MRJ Mason, JN Noordermee, LG Fradkin, MR Tannemaat, MJA Malessy, J Verhaagen, F De Winter Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury PLoS ONE, 2021;16(4):e0249748. 2021 [PMID: 33848304] (IHC, Rat)
• CM Lund, A Dyhl-Polk, DL Nielsen, LB Riis Wnt5a expression and prognosis in stage II-III colon cancer Transl Oncol, 2020;14(1):100892. 2020 [PMID: 33045677] (IHC, Human)
• W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32597829] (Western Blot, Mouse)
• S Ikezu, H Yeh, JC Delpech, ME Woodbury, AA Van Enoo, Z Ruan, S Sivakumara, Y You, C Holland, T Guillamon-, A Yoshii-Kit, MB Botros, C Madore, PH Chao, A Desani, S Manimaran, SV Kalavai, WE Johnson, O Butovsky, M Medalla, JI Luebke, T Ikezu Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities Mol. Psychiatry, 2020;0(0):. 2020 [PMID: 32071385] (ELISA Detection, Mouse)
• X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors Cells, 2019;8(10):. 2019 [PMID: 31557955] (IHC-Fr, Mouse)
• C Han, J Li, C Wang, H Ouyang, X Ding, Y Liu, S Chen, L Luo Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway Invest. Ophthalmol. Vis. Sci., 2018;59(8):3449-3460. 2018 [PMID: 30025083] (IHC, Mouse)
• K Glaeser, M Urban, E Fenech, O Voloshanen, D Kranz, F Lari, JC Christians, M Boutros ERAD-dependent control of the Wnt secretory factor Evi EMBO J., 2018;37(4):. 2018 [PMID: 29378775] (Western Blot, Human)
• J Xu, X Li, A Cole, Z Sherman, W Du Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells Stem Cells, 2018;0(0):. 2018 [PMID: 29377497] (Western Blot, Mouse)
• CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E619-E628. 2017 [PMID: 28069946] (Western Blot, Mouse)
• Hoeke A Baarsma Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 2016;0(0):. 2016 [PMID: 27979969] (Neutralization, Mouse)
• Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development Sci Rep, 2016;6(0):32094. 2016 [PMID: 27561676] (Neutralization, Human)
• Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 2016;0(0):. 2016 [PMID: 27402827] (Bioassay, N/A)
• IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling PLoS ONE, 2016;11(5):e0156002. 2016 [PMID: 27214384] (IHC, Human)
• T Skaria, E Bachli, G Schoedon Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells Cell Adh Migr, 2017;11(1):24-38. 2017 [PMID: 27159116] (ICC, Human)
• Bruce F Kimler WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation J. Endocrinol., 2016;229(2):197-207. 2016 [PMID: 26975616] (IHC-P, Rat)
• Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer, 2014;13(0):88. 2014 [PMID: 24766647] (Western Blot, Human)
• Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin. PLoS ONE, 2014;9(4):e94801. 2014 [PMID: 24728340] (Western Blot, Human)
• Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol, 2014;385(2):291-303. 2014 [PMID: 24211762] (Western Blot, Rat)
• Florian M, Nattamai K, Dorr K, Marka G, Uberle B, Vas V, Eckl C, Andra I, Schiemann M, Oostendorp R, Scharffetter-Kochanek K, Kestler H, Zheng Y, Geiger H A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature, 2013;503(7476):392-6. 2013 [PMID: 24141946] (IHC, Mouse)
• Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J. Cell. Sci., 2102;125(0):2017-29. 2102 [PMID: 22328498] (Western Blot, Mouse)
• NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway. Mol Brain, 2012;5(0):1. 2012 [PMID: 22217152] (Western Blot, Mouse)
• Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity. PLoS ONE, 2011;6(8):e22920. 2011 [PMID: 21857966] (Western Blot, Mouse)
• Bitler BG, Nicodemus JP, Li H, Cai Q, Wu H, Hua X, Li T, Birrer MJ, Godwin AK, Cairns P, Zhang R Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence. Cancer Res., 2011;71(19):6184-94. 2011 [PMID: 21816908] (Western Blot, Human)
• Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010;130(7):1849-59. 2010 [PMID: 20376066] (Bioassay, Human)
• Bodmer D, Levine-Wilkinson S, Richmond A, Hirsh S, Kuruvilla R Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons. J. Neurosci., 2009;29(23):7569-81. 2009 [PMID: 19515925] (Western Blot, Mouse)
• Christman MA, Goetz DJ, Dickerson E, McCall KD, Lewis CJ, Benencia F, Silver MJ, Kohn LD, Malgor R Wnt5a is expressed in murine and human atherosclerotic lesions. Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2864-70. 2008 [PMID: 18456733] (IHC-P, Human)
• Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler. Thromb. Vasc. Biol., 2008;28(3):504-10. 2008 [PMID: 18174455] (ICC, Human)
• Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G Dickkopf-1 is a master regulator of joint remodeling. Nat. Med., 2007;13(2):156-63. 2007 [PMID: 17237793] (Western Blot, Human)
• Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol. Biol. Cell, 2006;17(12):5163-72. 2006 [PMID: 17035633] (IHC, Mouse)
• Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation. Blood, 2006;108(3):965-73. 2006 [PMID: 16601243] (Neutralization, Human)
• Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion. Mol. Cell. Neurosci., 2005;31(2):251-62. 2005 [PMID: 16243537] (ICC, Mouse)
• Polesskaya A, Seale P, Rudnicki MA Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration. Cell, 2003;113(7):841-52. 2003 [PMID: 12837243] (IHC, Mouse)
• Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo. Proc. Natl. Acad. Sci. U.S.A., 2003;100(6):3422-7. 2003 [PMID: 12626754] (Immunodepletion, Xenopus)
• Ahumada A, Slusarski DC, Liu X, Moon RT, Malbon CC, Wang HY Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP. Science, 2002;298(5600):2006-10. 2002 [PMID: 12471263] (Immunodepletion, Mouse)

Reviews for Wnt-5a Antibody (AF645) (5)

Average Rating: 3.6

(Based on 5 reviews)

We have 5 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Anonymous - WB Human 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22547701
• Species: Human

reviewed by: Anonymous - IHC-P Human 01/09/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22973420
• Species: Human

reviewed by: Anonymous - IHC-Fr Mouse 01/09/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23029304
• Species: Mouse

reviewed by: Anonymous - IHC-P Rat 01/09/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24314182
• Species: Rat

reviewed by: Anonymous - IHC-P Human 01/09/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22547701
• Species: Human

Bioinformatics

• Gene Symbol: WNT5A
• Uniprot:
  • P22725()