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Wnt-3a Antibody (3A6)

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Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat ...read more
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium ...read more
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
3A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-19050AF350
Alexa Fluor 405
NBP1-19050AF405
Alexa Fluor 488
NBP1-19050AF488
Alexa Fluor 532
NBP1-19050AF532
Alexa Fluor 594
NBP1-19050AF594
Alexa Fluor 647
NBP1-19050AF647
Alexa Fluor 700
NBP1-19050AF700
Alexa Fluor 750
NBP1-19050AF750
Biotin
NBP1-19050B
DyLight 350
NBP1-19050UV
DyLight 405
NBP1-19050V
DyLight 488
NBP1-19050G
DyLight 550
NBP1-19050R
DyLight 594
NBP1-19050DL594
DyLight 650
NBP1-19050C
DyLight 680
NBP1-19050FR
DyLight 755
NBP1-19050IR
FITC
NBP1-19050F
HRP
NBP1-19050H
Janelia Fluor 549
NBP1-19050JF549
Janelia Fluor 646
NBP1-19050JF646
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Wnt-3a Antibody (3A6) Summary

Immunogen
Recombinant human Wnt-3a (19-352aa) purified from E. coli (NP_149122).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WNT3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100SDS-PAGE 1:100-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26708597)
Publications
Read Publication using
NBP1-19050 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Wnt-3a Antibody (3A6)

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
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Recombinant Human Wnt‑3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-1 (Catalog #5439‑DK/CF) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED50 for this effect is 10‑60 ng/mL (orange circles).
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Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.
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Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
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Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED50 is 1.7-fold better with more than twice the maximum response compared to the top competitor's Wnt-3a.
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Publications for Wnt-3a Antibody (NBP1-19050)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
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FAQs for Wnt-3a Antibody (NBP1-19050) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-19050AF350
Alexa Fluor 405 NBP1-19050AF405
Alexa Fluor 488 NBP1-19050AF488
Alexa Fluor 532 NBP1-19050AF532
Alexa Fluor 594 NBP1-19050AF594
Alexa Fluor 647 NBP1-19050AF647
Alexa Fluor 700 NBP1-19050AF700
Alexa Fluor 750 NBP1-19050AF750
Biotin NBP1-19050B
DyLight 350 NBP1-19050UV
DyLight 405 NBP1-19050V
DyLight 488 NBP1-19050G
DyLight 550 NBP1-19050R
DyLight 594 NBP1-19050DL594
DyLight 650 NBP1-19050C
DyLight 680 NBP1-19050FR
DyLight 755 NBP1-19050IR
FITC NBP1-19050F
HRP NBP1-19050H
Janelia Fluor 549 NBP1-19050JF549
Janelia Fluor 646 NBP1-19050JF646
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Bioinformatics

Gene Symbol WNT3A
Entrez
Uniprot