Wnt-3a Antibody (3A6) - BSA Free

Images

 
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat ...read more
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium ...read more
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Wnt-3a Antibody (3A6) - BSA Free Summary

Immunogen
Recombinant human Wnt-3a (19-352aa) purified from E. coli (NP_149122).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WNT3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100SDS-PAGE 1:100-1:2000
  • Western Blot 1:1000-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26708597)
Publications
Read Publications using
NBP1-19050 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Wnt-3a Antibody (3A6) - BSA Free

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Anti-human Wnt3a mAb, clone 3A6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Wnt3a protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Wnt-3a Antibody (NBP1-19050)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-19050 Applications Species
Denysenko T, Annovazzi L, Cassoni P et al. WNT/b-catenin Signaling Pathway and Downstream Modulators in Low- and High-grade Glioma. Cancer Genomics Proteomics. 2015-12-28 [PMID: 26708597] (WB, ICC/IF, IHC-P, Human) WB, ICC/IF, IHC-P Human
Sheng J Cellular Effects Nanosilver on Cancer and Non-cancer Cells: Potential Environmental and Human Health Impacts Thesis
Cameron S Anti-Cancer and Stress Response Pathway Effects of Nanosilver and Sodium Ascorbate Carleton University 2022-07-11 (WB, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol WNT3A
Entrez
Uniprot