Wnt-10b Antibody (HL2370) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Wnt10b stained by Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Wnt10b stained by Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Wnt-10b Antibody (HL2370) - Azide and BSA Free [NBP3-25749] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (NBP3-25749) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, IHC
• Clone: HL2370
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Wnt-10b Antibody (HL2370) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Wnt-10b.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WNT10B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Wnt-10b Antibody (HL2370) - Azide and BSA Free

• protein Wnt-10b
• Protein Wnt-12
• SHFM6WNT-10B protein
• wingless-type MMTV integration site family, member 10B
• Wnt10b
• Wnt-10b
• WNT12
• Wnt-12

Background

Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. Novus is pleased to offer researchers access to products that are not fully characterized or validated. This antibody is shown by Peptide ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest. * We cannot guarantee that this antibody will work in any application other than in a Peptide ELISA against the peptide used as immunogen and therefore can not offer a refund if the antibody does not work in your application. * The antibody is offered at a lower price compared to more highly validated antibodies. * We are not able to provide the peptide used as immunogen for this product. As this antibody is not fully validated we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.__________________________ Wnts comprise a family of secreted signaling proteins that regulate diverse developmental processes. Activation of Wnt signaling by Wnt10b inhibits differentiation of preadipocytes and blocks adipose tissue development. In bone development, Wnt10b shifts cell fate toward the osteoblast lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT10B