Wnt-10a Antibody

Images

 
Western Blot: Wnt-10a Antibody [NBP1-76916] - RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Wnt-10a Antibody [NBP1-76916] - Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP1-76916] - Staining of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP1-76916] - Human skeletal muscle tissue cells with Wnt10a antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Wnt-10a Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. The immunogen is located within amino acids 300 - 350 of Wnt10a. Amino Acid Squence: APGAPGPRRRASPA
Specificity
This Wnt10a antibody is predicted to not cross-react with Wnt10b.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WNT10A
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Wnt-10a Peptide (NBP1-76916PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Wnt-10a Antibody

  • FLJ14301
  • protein Wnt-10a
  • SSPS
  • wingless-type MMTV integration site family, member 10A
  • Wnt10a
  • Wnt-10a

Background

Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia. Despite the high homology of Wnt10a to Wnt10b, this Wnt10a antibody will not cross-react with Wnt10b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Calculators

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Bioinformatics

Gene Symbol WNT10A
Uniprot