WNK1 Antibody [Unconjugated]

Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat WNK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2849) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WNK1 at approximately 230 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

WNK1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat WNK1
  Ser816-Ser1173
  Accession # Q9JIH7
• Specificity: Detects mouse and rat WNK1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNK1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WNK1 Antibody [Unconjugated]

• EC 2.7.11
• EC 2.7.11.1
• Erythrocyte 65 kDa protein
• HSAN2hereditary sensory neuropathy, type II
• HSN2MGC163339
• hWNK1
• KDP
• KDPMGC163341
• KIAA0344
• Kinase deficient protein
• p65
• PHA2C
• PRKWNK1
• PRKWNK1PSK
• prostate-derived sterile 20-like kinase
• Protein kinase lysine-deficient 1
• Protein kinase with no lysine 1
• protein kinase, lysine deficient 1
• serine/threonine-protein kinase WNK1
• WNK lysine deficient protein kinase 1
• WNK1

Background

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WNK1 Antibody (AF2849)(6)

Publications using AF2849

• Ji-Ung J, Steve S, Melanie H C Activation of WNK1 signaling through Piezo1 Proc Natl Acad Sci U S A. 2025-08-29 [PMID: 40880539] (Westen Blot, Immunoprecipitation, Human)
• Trzeciak, AJ;Rojas, WS;Liu, ZL;Krebs, AS;Wang, Z;Saavedra, PHV;Miranda, IC;Lipshutz, A;Xie, J;Huang, CL;Overholtzer, M;Glickman, MS;Parkhurst, CN;Vierbuchen, T;Lucas, CD;Perry, JSA; WNK1 enforces macrophage lineage fidelity bioRxiv : the preprint server for biology 2023-04-28 [PMID: 37383948] (Western Blot, Mouse)
• Jin X, Xie J, Yeh CW et al. WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin release The Journal of clinical investigation 2023-04-18 [PMID: 37071482]
• B Saha, DCAL Dellova, J Demko, MV Sørensen, E Takagi, CE Gleason, W Shabbir, D Pearce WNK1 is a chloride-stimulated scaffold that regulates mTORC2-activity and ion transport Journal of Cell Science, 2022-12-05;0(0):. 2022-12-05 [PMID: 36373794] (Western Blot, Mouse)
• S Ishihara, T Sato, G Du, D Guardavacc, A Nakajima, S Sawai, T Kataoka, K Katagiri Phosphatidic acid-dependent localization and basal de-phosphorylation of RA-GEFs regulate lymphocyte trafficking BMC Biol., 2020-06-29;18(1):75. 2020-06-29 [PMID: 32600317] (Flow Cytometry, Mouse)
• Shekarabi M, Girard N, Riviere JB, Dion P, Houle M, Toulouse A, Lafreniere RG, Vercauteren F, Hince P, Laganiere J, Rochefort D, Faivre L, Samuels M, Rouleau GA Mutations in the nervous system--specific HSN2 exon of WNK1 cause hereditary sensory neuropathy type II. J. Clin. Invest., 2008-07-01;118(7):2496-505. 2008-07-01 [PMID: 18521183] (Western Blot, Mouse)

Review for WNK1 Antibody (AF2849) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence staining Human 12/07/2023

Summary

• Application: Other
• Sample Tested: Human Dermal Microvascular Endothelial Cells - fixed with PFA
• Species: Human
• Lot: VNP032311A

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: WNK1
• Uniprot:
  • Q9JIH7()