WISP-1/CCN4 Antibody

Western Blot: WISP-1/CCN4 Antibody [NB100-77339] - Staining of Caco-2 cell lysate 1 (A) and Caco-2 cell lysate 2 (B) + petide (C) with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: WISP-1/CCN4 Antibody [NB100-77339] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: WISP-1/CCN4 Antibody [NB100-77339] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: WISP-1/CCN4 Antibody [NB100-77339] - Staining of Human Tonsil lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: WISP-1/CCN4 Antibody [NB100-77339] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

WISP-1/CCN4 Antibody Summary

• Description: Novus Biologicals Goat WISP-1/CCN4 Antibody (NB100-77339) is a polyclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-WISP-1/CCN4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ESYPDFSEIAN corresponding to C-Terminus according to NP_543028.1.
• Epitope: ESYPDFSEIAN
• Specificity: This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCN4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated MW of 30.7 kDa according to NP_543028.1 and 40.3 kDa according to NP_003873.1).
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  WISP-1/CCN4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WISP-1/CCN4 Antibody

• CCN family member 4
• CCN4
• CCN4WISP1tc
• WISP1
• WISP-1
• WISP1c
• WISP1i
• WNT1 induced secreted protein 1
• WNT1 inducible signaling pathway protein 1
• Wnt-1 inducible signaling pathway protein 1
• wnt-1 signaling pathway protein 1
• Wnt-1-induced secreted protein
• WNT1-inducible-signaling pathway protein 1

Background

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WISP-1/CCN4 Antibody (NB100-77339)(1)

Publication using NB100-77339

• Major MB, Camp ND, Berndt JD et al. Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling. Science 2007-05-18 [PMID: 17510365]

Bioinformatics

• Gene Symbol: CCN4
• Entrez:
  • 8840(Human)
  • 22402(Mouse)
  • 65154(Rat)
• Uniprot:
  • O95388()