Detects mouse WISP‑1/CCN4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) WISP-1 is observed and less than 1% cross-reactivity with rhCTGF and recombinant mouse NOV is observed.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for WISP-1/CCN4 Antibody
CCN family member 4
WNT1 induced secreted protein 1
WNT1 inducible signaling pathway protein 1
Wnt-1 inducible signaling pathway protein 1
wnt-1 signaling pathway protein 1
Wnt-1-induced secreted protein
WNT1-inducible-signaling pathway protein 1
Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 66 aa von Willebrand factor type C (VWC) module (aa 121‑186), a 41 aa TSP type I domain (aa 220‑260) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7‑9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).
Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
Tanada, S. et al. (2001) Oncogene 20:5525.
Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
Brigstock, D.R. (2003) J. Endocrinol. 178:169.
Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
SwissProt Accession # O54775.
SwissProt Accession # Q99PP0.
SwissProt Accession # O95388
French, D.M. et al. (2004) Am. J. Pathol. 165:855.
Parisi, M.S. et al. (2006) Bone 38:671.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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