WISP-1/CCN4 Antibody

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WISP‑1/CCN4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Sheep Anti-Mouse WISP‑1/CCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1680) overnight at 4 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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WISP-1/CCN4 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse WISP‑1/CCN4
Thr23-Asn367
Accession # O54775
Specificity
Detects mouse WISP‑1/CCN4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) WISP-1 is observed and less than 1% cross-reactivity with rhCTGF and recombinant mouse NOV is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
WISP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF1680 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WISP-1/CCN4 Antibody

  • CCN family member 4
  • CCN4
  • CCN4WISP1tc
  • WISP1
  • WISP-1
  • WISP1c
  • WISP1i
  • WNT1 induced secreted protein 1
  • WNT1 inducible signaling pathway protein 1
  • Wnt-1 inducible signaling pathway protein 1
  • wnt-1 signaling pathway protein 1
  • Wnt-1-induced secreted protein
  • WNT1-inducible-signaling pathway protein 1

Background

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 66 aa von Willebrand factor type C (VWC) module (aa 121‑186), a 41 aa TSP type I domain (aa 220‑260) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7‑9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
  7. SwissProt Accession # O54775.
  8. SwissProt Accession # Q99PP0.
  9. SwissProt Accession # O95388
  10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol WISP1
Uniprot