WIPI2 Antibody - BSA Free

Western Blot: WIPI2 Antibody [NBP3-13110] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (NBP3-13110) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: WIPI2 Antibody [NBP3-13110] - WIPI2 antibody detects WIPI2 protein at Preautophagosomal structure membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: WIPI2 protein stained by WIPI2 antibody (NBP3-13110) diluted at 1:2500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP3-13110] - WIPI2 antibody detects WIPI2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. WIPI2 antibody (NBP3-13110) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: WIPI2 Antibody [NBP3-13110] - Various whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (NBP3-13110) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WIPI2 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human WIPI2. The exact sequence is proprietary.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WIPI2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for WIPI2 Antibody - BSA Free

• ATG18B
• Atg21
• CGI-50
• DKFZP434J154
• DKFZp686P02188
• FLJ12979
• FLJ14217
• FLJ42984
• WD repeat domain phosphoinositide-interacting protein 2
• WD repeat domain, phosphoinositide interacting 2
• WD40 repeat protein interacting with phosphoinositides 2
• WIPI-2
• WIPI49-like protein 2

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WIPI2