ARAP3 Antibody - BSA Free

Staining of human kidney shows moderate membranous-cytoplasmic positivity in cells in glomeruli and endothelial cells.

Staining of human placenta shows moderate membranous-cytoplasmic positivity in endothelial cells.

Staining of human lymphoid tissues shows moderate membranous-cytoplasmic positivity in endothelial cells.

Analysis in human cell line EFO-21.

Staining of human duodenum shows strong membranous positivity in endothelial cells and weak cytoplasmic membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARAP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARAP3 Antibody - BSA Free (NBP1-84541) is a polyclonal antibody validated for use in IHC and WB. Anti-ARAP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MPNSIYFGLDSRGRAQAAQDKAPDSSQISAPTPALRPTTGTVHIMDPGCLYYGVQPVGTPGAPDRRESRGVCQGRAEHRLSRQDLEAREDAGYASLELPGDSTLLSPTLETEETSDDLISPYASFSF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ARAP3 Protein (NBP1-84541PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ARAP3 Antibody - BSA Free

• Arf and Rho GAP adapter protein 3
• arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
• ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
• ARF-GAP, RHO-GAP, ankyrin repeat and plekstrin homology domains-containingprotein 3
• centaurin, delta 3
• centaurin-delta-3
• CENTD3
• cnt-d3
• DRAG1
• FLJ21065
• phosphoinositide binding protein
• PtdIns(34,5)P3-binding protein

Background

The ARAP3 gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrinhomology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton andcell shape. It is a specif

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARAP3 Antibody (NBP1-84541)(1)

Publication using NBP1-84541

• Posor Y, Kampyli C, Bilanges B et al. Local synthesis of the phosphatidylinositol-3,4-bisphosphate lipid drives focal adhesion turnover Developmental cell 2022-07-25 [PMID: 35809565] (WB, Human)

Bioinformatics

• Gene Symbol: ARAP3
• Entrez:
  • 64411(Human)
• Uniprot:
  • Q8WWN8(Human)