WDR62 Antibody - BSA Free

Immunoprecipitation: WDR62 Antibody [NB100-77302] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR62 antibody NB100-77302 used for IP at 3 ug/mg lysate. WDR62 was also immunoprecipitated by rabbit anti WDR62 antibody NB100-77303 which recognizes a downstream epitope. For blotting immunoprecipitated WDR62, NB100-77303 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

WDR62 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human WD repeat domain 62 using the numbering given in entry NP_775907.4 (GeneID 284403).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WDR62
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:100-1:2000
• Application Notes: ICC/IFand WB were reported in scientific literature (PMID: 22899712).

Reactivity Notes

Mouse reactivity reported in (PMID: 22899712).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WDR62 Antibody - BSA Free

• C19orf14MGC166976
• chromosome 19 open reading frame 14
• DKFZP434J046
• DKFZp686G1024
• EC 2.7.11.1
• EC 2.7.7.38
• FLJ33298
• WD repeat domain 62
• WD repeat-containing protein 62

Background

WDR62 (WD repeat-containing 62) contains 15 WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. WDR62 has been identified in a phosphoproteome analysis as a protein associated with the mitotic spindle; however its function has not been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WDR62 Antibody (NB100-77302)(1)

Publication using NB100-77302

• Bogoyevitch MA, Yeap YY, Qu Z et al. WD40-repeat protein 62 is a JNK-phosphorylated spindle pole protein required for spindle maintenance and timely mitotic progression. J Cell Sci 2012-11-01 [PMID: 22899712] (ICC/IF, WB, Mouse)

Bioinformatics

• Gene Symbol: WDR62
• Entrez:
  • 284403(Human)
• Uniprot:
  • O43379(Human)