JIP1 Antibody

Western blot shows lysates of C2C12 mouse myoblast cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat JIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4366) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for JIP1 at approximately 105 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

JIP1 Antibody Summary

• Immunogen: E. coli-derived recombinant human JIP1
  Met531-Asp666
  Accession # Q6NUQ9
• Specificity: Detects endogenous human, mouse, and rat JIP1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MAPK8IP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JIP1 Antibody

• C-Jun-amino-terminal kinase-interacting protein 1;IB1;JIP1;JIP-1;MAPK8IP1;PRKM8IP
• IB1
• JIP1
• MAPK8IP1
• PRKM8IP

Background

JNK-interacting proteins (JIPs) comprise a family of four scaffolding proteins that tether and assemble components of the JNK signaling cascade. JIP1, also known as islet-brain 1 (IB1) and mitogen-activated protein kinase 8-interacting protein 1 (MAPK8IP1), contains an N-terminal JNK-binding domain, and C-terminal MLK- and MKK7-binding domains. JIP1 is ubiquitously expressed, with highest levels in brain and in pancreatic beta -cells. Mice lacking JIP1 are resistant to diet-induced obesity and show reduced diet-induced insulin resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JIP1 Antibody (AF4366)(3)

Publications using AF4366

• Cason SE, Carman PJ, Van Duyne C et al. Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway Journal of Cell Biology 2021-07-05 [PMID: 34014261]
• Fu M, Nirschl J, Holzbaur E LC3 binding to the scaffolding protein JIP1 regulates processive dynein-driven transport of autophagosomes. Dev Cell, 2014-06-09;29(5):577-90. 2014-06-09 [PMID: 24914561] (ICC, Mouse)
• M. Vieira, J. Fernandes, A. Burgeiro, G.M. Thomas, R.L. Huganir, C.B. Duarte et al. Excitotoxicity through Ca2+-permeable AMPA receptors requires Ca2+-dependent JNK activation Neurobiology of Disease 2010-12-01 [PMID: 20708684]

Bioinformatics

• Gene Symbol: MAPK8IP1
• Uniprot:
  • Q6NUQ9()