WASF1/WAVE1 [p Tyr125] Antibody

Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WASF1/WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western Blot: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WASF1/WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

WASF1/WAVE1 [p Tyr125] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-WASF1/WAVE1 (Tyr-125) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-125 in human WASF1/WAVE1. This sequence has high homology with similar regions in rat and mouse WASF1/WAVE1, and has less than 50% homology to similar regions in the conserved site in WAVE2 (Tyr-124) and WAVE3 (Tyr-125). [UniProt# Q92558]
• Modification: p Tyr125
• Specificity: This antibody was cross-adsorbed to phospho-WASF1/WAVE1 (Tyr-150) and unphosphorylated WASF1/WAVE1 (Tyr-125) peptides before affinity purification using phospho-WASF1/WAVE1 (Tyr-125) peptide. In western blots, the antibody detects an 80 kDa band corresponding to phosphorylated WASF1/WAVE1 in human SYF cSrc transformed cells and mouse macrophages treated with pervanadate, but is not observed in control cells. These bands can be specifically blocked with phospho-WASF1/WAVE1 (Tyr-125) peptide (WX1775), but not phospho-WASF1/WAVE1 (Tyr-150) peptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WASF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:1000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for WASF1/WAVE1 [p Tyr125] Antibody

• KIAA0269WAVEhomology of dictyostelium scar 1
• Protein WAVE-1
• SCAR1
• SCAR1WASP family protein member 1
• Verprolin homology domain-containing protein 1
• WAS protein family, member 1
• WASF1
• WASP1
• WAVE1
• WAVE1FLJ31482
• wisk

Background

WASP (for Wiskott-Aldrich syndrome protein), N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WASF1