HAF109). A specific band was detected for WASF1/WAVE1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A172 human glioblastoma cell line, and 293T human embryonic kidney cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF1/WAVE1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5514) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF1/WAVE1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5514-SP | |||
![PPP2R1A interacts with the WAVE Shell Complex (WSC) that contains NHSL1 instead of WAVE proteins.a MCF10A or MDA-MB-231 cells stably expressing FLAG-GFP or FLAG-GFP PPP2R1A were subjected to FLAG-GFP Tandem Affinity Purification and purified proteins were resolved by SDS–PAGE and silver stained. The same samples were used for mass spectrometry and Western blots. Three biological repeats with similar results. b Label-free quantification (LFQ) of proteins identified by mass spectrometry. In both cell lines, NHSL1 is present at the expense of WAVE subunits. Log10 of LFQ values, mean ± sem of three biological repeats. c GFP immunoprecipitates were analyzed by Western blots with the indicated antibodies. Three biological repeats with similar results. d PPP2R1A associates with the WSC through NHSL1. MCF10A cells expressing FLAG-GFP PPP2R1A were transfected with pools of siRNAs targeting NHSL1 or non-targeting siRNAs (CTRL). GFP immunoprecipitates were analyzed by western blots. Three biological repeats with similar results. e A MCF10A cell line stably transfected with two plasmids expressing FLAG-GFP PPP2R1A and PC-HA BRK1 was subjected to FLAG-PC Tandem Affinity Purification to purify the WSC. WSC composition was analyzed by mass spectrometry. Silver stained SDS–PAGE of purified WSC. The identity of WSC subunits and the lack of WAVE2 was confirmed using Western blots. 3 biological repeats with similar results. f Comparison of the structural model of the WSC obtained using AlphaFold2 with the X-ray crystal structure WAVE Regulatory Complex (PDB:3P8C [//www.wwpdb.org/pdb?id=pdb_00003p8c]). The WSC was composed of NHSL1 (1-123) in purple, CYFIP1 in green, NCKAP1 in cyan, BRK1 in yellow and ABI2 (1–160) in salmon. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37322026), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af5514_human-mouse-wasf1-wave1-affinity-purified-polyclonal-ab-western-blot-12122025923169.jpg)
![PPP2R1A interacts with the WAVE Shell Complex (WSC) that contains NHSL1 instead of WAVE proteins.a MCF10A or MDA-MB-231 cells stably expressing FLAG-GFP or FLAG-GFP PPP2R1A were subjected to FLAG-GFP Tandem Affinity Purification and purified proteins were resolved by SDS–PAGE and silver stained. The same samples were used for mass spectrometry and Western blots. Three biological repeats with similar results. b Label-free quantification (LFQ) of proteins identified by mass spectrometry. In both cell lines, NHSL1 is present at the expense of WAVE subunits. Log10 of LFQ values, mean ± sem of three biological repeats. c GFP immunoprecipitates were analyzed by Western blots with the indicated antibodies. Three biological repeats with similar results. d PPP2R1A associates with the WSC through NHSL1. MCF10A cells expressing FLAG-GFP PPP2R1A were transfected with pools of siRNAs targeting NHSL1 or non-targeting siRNAs (CTRL). GFP immunoprecipitates were analyzed by western blots. Three biological repeats with similar results. e A MCF10A cell line stably transfected with two plasmids expressing FLAG-GFP PPP2R1A and PC-HA BRK1 was subjected to FLAG-PC Tandem Affinity Purification to purify the WSC. WSC composition was analyzed by mass spectrometry. Silver stained SDS–PAGE of purified WSC. The identity of WSC subunits and the lack of WAVE2 was confirmed using Western blots. 3 biological repeats with similar results. f Comparison of the structural model of the WSC obtained using AlphaFold2 with the X-ray crystal structure WAVE Regulatory Complex (PDB:3P8C [//www.wwpdb.org/pdb?id=pdb_00003p8c]). The WSC was composed of NHSL1 (1-123) in purple, CYFIP1 in green, NCKAP1 in cyan, BRK1 in yellow and ABI2 (1–160) in salmon. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37322026), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af5514_human-mouse-wasf1-wave1-affinity-purified-polyclonal-ab-western-blot-1212202597424.jpg)
![Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WASF1/WAVE1 stained by WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13180-img0005.jpg "Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WASF1/WAVE1 stained by WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-Immunohistochemistry-Paraffin-NBP3-13180-img0004.jpg)
![Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-Immunohistochemistry-Paraffin-NBP3-13180-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WASF1/WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23190_rabbit-wasf1-wave1-p-tyr125-pab-199202312523625.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WASF1/WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![<b>Biological Strategies Validation. </b>Western Blot: WASF1/WAVE1 [p Tyr125] Antibody [NBP3-23190] - Western blot of human SYF cSrc transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM; 30 min). The blots were probed with anti-WASF1/WAVE1 (N-terminal region) (lanes 1 & 2) or anti-WAVE (Tyr-125) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23190_rabbit-wasf1-wave1-p-tyr125-pab-19920231856928.jpg)
![Western Blot: WASF1/WAVE1 Antibody [NBP3-23363] - Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WASF1/WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WASF1/WAVE1 (N-terminal region) peptide (lane 5) or WASF2 (Central region) peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23363_rabbit-wasf1-wave1-pab-19920231915411.jpg "Western Blot: WASF1/WAVE1 Antibody [NBP3-23363] - Western blot of human SYF cSrc-transformed cells. Blots were were probed with anti-WASF1/WAVE1 (N-terminal region) at a dilution of 1:1000 (lane 1), 1:2000 (lane 2) or 1:4000 (lane 3). In addition, the antibody was used in the absence (lane 4) or presence of blocking peptides, WASF1/WAVE1 (N-terminal region) peptide (lane 5) or WASF2 (Central region) peptide (lane 6).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-23363] - Immunocytochemical labeling of phosphorylated WAVE in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal WASF1/WAVE1 (N-terminal region) and WAVE (Tyr-125) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23363_rabbit-wasf1-wave1-pab-199202317594421.jpg)
![Western Blot: WASF1/WAVE1 Antibody [NBP1-83018] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HEL](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-Western-Blot-NBP1-83018-img0003.jpg "Western Blot: WASF1/WAVE1 Antibody [NBP1-83018] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HEL")
![Immunohistochemistry: WASF1/WAVE1 Antibody [NBP1-83018] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-Immunohistochemistry-NBP1-83018-img0002.jpg)
![Western Blot: WASF1/WAVE1 Antibody (S03-5K1) [NBP3-15110] - Western blot detection of WASF1/WAVE1 in Rat Brain, C6, 3T3, Hela cell lysates using WASF1/WAVE1 Rabbit mAb (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-S03-5K1-Western-Blot-NBP3-15110-img0001.jpg "Western Blot: WASF1/WAVE1 Antibody (S03-5K1) [NBP3-15110] - Western blot detection of WASF1/WAVE1 in Rat Brain, C6, 3T3, Hela cell lysates using WASF1/WAVE1 Rabbit mAb (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 90kDa.")
![SDS-Page: Recombinant Human WASF1/WAVE1 Protein [H00008936-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00008936-Q01-1.jpg "SDS-Page: Recombinant Human WASF1/WAVE1 Protein [H00008936-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: WASF1 Overexpression Lysate (Adult Normal) [NBL1-17775] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WASF1.](http://images.novusbio.com/fullsize/WASF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17775-img0002.jpg "Western Blot: WASF1 Overexpression Lysate (Adult Normal) [NBL1-17775] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WASF1.")
![Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP2-88584] - Rabbit Anti-WASF1 antibody. Catalog Number: ARP63994. Formalin Fixed Paraffin Embedded Tissue: Human Brain, Cortex. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification:](http://images.novusbio.com/fullsize/WASF1-WAVE1-Antibody-Immunohistochemistry-Paraffin-NBP2-88584-img0001.jpg "Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP2-88584] - Rabbit Anti-WASF1 antibody. Catalog Number: ARP63994. Formalin Fixed Paraffin Embedded Tissue: Human Brain, Cortex. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification:")
