CYFIP1 Antibody

Images

 
Western Blot: CYFIP1 Antibody [NBP2-16060] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CYFIP1 Antibody [NBP2-16060] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: CYFIP1 Antibody [NBP2-16060] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Western Blot: CYFIP1 Antibody [NBP2-16060] - Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE gel, diluted at 1:1000.
Immunoprecipitation: CYFIP1 Antibody [NBP2-16060] - Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 ug of CYFIP1 antibody [N1N2], N-term. Western blot analysis was performed using CYFIP1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CYFIP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human CYFIP1. The exact sequence is proprietary.
Localization
Cytoplasm; perinuclear region; Cell projection; lamellipodium; ruffle; Cell junction; synapse; synaptosome
Predicted Species
Rat (100%), Bovine (100%), Zebrafish (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYFIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CYFIP1 Antibody

  • cytoplasmic FMR1 interacting protein 1
  • cytoplasmic FMR1-interacting protein 1
  • FLJ45151
  • KIAA0068cytoplasmic FMRP interacting protein 1
  • P140SRA-1
  • selective hybridizing clone
  • SHYC
  • Specifically Rac1-associated protein 1
  • SRA1
  • sra-1

Background

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CYFIP1
Uniprot