WASF1/WAVE1 Antibody

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Western Blot: WASF1/WAVE1 Antibody [NBP3-13180] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WASF1/WAVE1 antibody (NBP3-13180) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed ...read more
Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ...read more
Immunocytochemistry/ Immunofluorescence: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% ...read more
Immunohistochemistry-Paraffin: WASF1/WAVE1 Antibody [NBP3-13180] - WASF1/WAVE1 antibody detects WASF1/WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

WASF1/WAVE1 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse WASF1/WAVE1. The exact sequence is proprietary.
Predicted Species
Bovine (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WASF1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for WASF1/WAVE1 Antibody

  • KIAA0269WAVEhomology of dictyostelium scar 1
  • Protein WAVE-1
  • SCAR1
  • SCAR1WASP family protein member 1
  • Verprolin homology domain-containing protein 1
  • WAS protein family, member 1
  • WASF1
  • WASP1
  • WAVE1
  • WAVE1FLJ31482
  • wisk

Background

WASP (for Wiskott-Aldrich syndrome protein), N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol WASF1