VPS36 Antibody - BSA Free

Western Blot: VPS36 Antibody [NBP2-20877] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: VPS36 Antibody [NBP2-20877] - Paraffin-embedded mouse intestine. VPS36 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: VPS36 Antibody [NBP2-20877] - Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VPS36 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human VPS36. The exact sequence is proprietary.
• Predicted Species: Rat (95%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS36
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (86%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VPS36 Antibody - BSA Free

• C13orf9
• CGI-145
• DKFZp781E0871
• Eap45
• EAP45chromosome 13 open reading frame 9
• ELL-associated protein of 45 kDa
• ELL-associated protein, 45 kDa
• ESCRT-II complex subunit VPS36
• vacuolar protein sorting 36 homolog (S. cerevisiae)
• vacuolar protein sorting 36 homolog (yeast)
• vacuolar protein-sorting-associated protein 36

Background

VPS36, VPS22 (SNF8; MIM 610904), and VPS25 (MIM 610907) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS36, VPS22, and VPS25 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: VPS36
• Uniprot:
  • Q86VN1(Human)