VPS25 Antibody (2E5-2B9) - Azide and BSA Free

Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01] - MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Analysis of MGC10540 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Analysis of monoclonal antibody to VPS25 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2E5-2B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VPS25 Antibody (2E5-2B9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse VPS25 Antibody (2E5-2B9) - Azide and BSA Free (H00084313-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MGC10540 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
• Specificity: VPS25 - hypothetical protein MGC10540
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VPS25
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VPS25 Antibody (2E5-2B9) - Azide and BSA Free

• Dermal papilla-derived protein 9
• DERP9
• DERP9hVps25
• EAP20
• EAP20FAP20
• ELL-associated protein of 20 kDa
• ESCRT-II complex subunit VPS25
• FAP20
• MGC10540
• vacuolar protein sorting 25 (yeast)
• vacuolar protein sorting 25 homolog (S. cerevisiae)
• vacuolar protein-sorting-associated protein 25

Background

VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS25
• Entrez:
  • 84313(Human)
• Uniprot:
  • AAH06282(Human)