VprBP Antibody

Western Blot: VprBP Antibody [NBP1-05953] - Whole cell lysate from HeLa and 293T (T; 50 ug) cells. VprBP was also immunoprecipitated by another rabbit anti-VPRBP antibody NBP1-05952.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

VprBP Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1457 and 1507 of human Vpr (HIV-1) binding protein using the numbering given in entry NP_055518.1 (GeneID 9730).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPRBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VprBP Antibody

• DCAF1DDB1 and CUL4 associated factor 1
• DDB1- and CUL4-associated factor 1
• HIV-1 Vpr-binding protein
• KIAA0800protein VPRBP
• MGC102804
• RIP
• Vpr (HIV-1) binding protein
• Vpr-binding protein
• VprBP
• Vpr-interacting protein

Background

VprBP (HIV-1 Vpr-binding protein) is also known as DCAF1 (Ddb1- and Cul4-associated factor 1). VprBP was initially identified as a protein that co-immunoprecipitated with the HIV protein, viral protein R; however the physiological relevance of this association remains unclear. It has recently been shown that VprBP plays an essential role in cell cycle progression, and it associates with the DDB1-CUL4 E3 ligase complex. Alternate names for VprBP include Vpr-interacting protein, RIP, and KIAA0800.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VprBP Antibody (NBP1-05953)(2)

Publications using NBP1-05953

• Britschgi A, Duss S, Kim S et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERa Nature 2017-01-26 [PMID: 28068668]
• Salsman J, Jagannathan M, Paladino P et al. Proteomic profiling of the human cytomegalovirus UL35 gene products reveals a role for UL35 in the DNA repair response. J Virol 2012-01-01 [PMID: 22072767]

Bioinformatics

• Gene Symbol: VPRBP
• Entrez:
  • 9730(Human)
• Uniprot:
  • Q9Y4B6(Human)