Von Willebrand Factor Antibody (F8/86)

Product Discontinued

Von Willebrand Factor Antibody (F8/86) Summary

• Immunogen: Von Willebrand factor isolated from human plasma.
• Localization: Cytoplasmic
• Marker: Endothelial Cell Marker
• Specificity: This reacts with Von Willebrand factor in endothelial cells. It also reacts with megakaryocytes in human bone marrow. Proteolytic enzyme digestion of formalin fixed paraffin embedded tissue sections is required prior to immunostaining.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VWF
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Von Willebrand Factor Antibody (F8/86)

• coagulation factor VIII VWF
• F8
• F8VWF
• von Willebrand factor
• VWD
• vWF

Background

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Von Willebrand Factor Antibody (NBP1-22620). (Showing 1 - 1 of 1 FAQ).

1. We have paraffin embedded mouse tissue implanted with human cells. We would like to differentiate between the mouse and human blood vessels in those sections with immunohistochemistry. So we need an antibody that binds human but not mouse endothelial. I see that you have a Factor VIII Antibody (NBP1-22620) that seems interesting. Have the cross reactivity of this antibody been tested against mouse? Can you recommend this antibody for our use?
   • I have taken a look at this antibody and I would certainly not recommend this as the host species is mouse therefore there will be a lot of back ground staining as cross reactivity will be high. I have carried out a search and unfortunately we do not have an antibody that will not cross react with mouse. I am very sorry that we do not have what you are looking for.

Bioinformatics

• Gene Symbol: VWF