Human Serpin E1/PAI-1 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not... detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.
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• Specificity: Natural and recombinant human Serpin E1
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: SERPINE1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Serpin E1/PAI-1 Quantikine ELISA Kit

• Endothelial plasminogen activator inhibitor
• Nexin
• PAI1
• PAI-1
• PAI1PAI-1
• PAISerpin E1
• PLANH1
• PLANH1plasminogen activator inhibitor 1
• serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
• Serpin E1
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Serpin E1/PAI-1 (DSE100)(38)

Publications using DSE100

• KD Wick, L Siegel, JD Neaton, C Oldmixon, J Lundgren, RL Dewar, HC Lane, BT Thompson, MA Matthay, ACTIV-3/TI RAGE has potential pathogenetic and prognostic value in nonintubated hospitalized patients with COVID-19 JCI Insight, 2022;7(9):. 2022 [PMID: 35298440] (Human)
• Y Qin, J Zhang, S Babapoor-F, B Applewhite, M Deshpande, H Megarity, M Flores-Bel, S Aparicio-D, T Ma, Y Rui, SY Tzeng, JJ Green, MV Canto-Sole, S Montaner, A Sodhi PAI-1 is a vascular cell-specific HIF-2-dependent angiogenic factor that promotes retinal neovascularization in diabetic patients Science Advances, 2022;8(9):eabm1896. 2022 [PMID: 35235351] (Human)
• C Cohen, O Le Goff, F Soysouvanh, F Vasseur, M Tanou, C Nguyen, L Amrouche, J Le Guen, O Saltel-Ful, T Meunier, T Nguyen-Kho, M Rabant, D Nochy, C Legendre, G Friedlande, BG Childs, DJ Baker, B Knebelmann, D Anglicheau, F Milliat, F Terzi Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1 Embo Molecular Medicine, 2021;13(11):e14146. 2021 [PMID: 34725920] (Human)
• M McAleavy, Q Zhang, PJ Ehmann, J Xu, MF Wipperman, D Ajithdoss, L Pan, M Wakai, R Simonson, A Gadi, A Oyejide, SC Hamon, A Boyapati, LG Morton, T Shavlakadz, CA Kyratsous, DJ Glass The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients Molecular and Cellular Biology, 2021;0(0):MCB0046721. 2021 [PMID: 34723652] (Human)
• SH Cho, JH Kim, HJ An, YR Kim, EH Ahn, JR Lee, JO Kim, JJ Ko, NK Kim Genetic Polymorphisms in miR-604A>G, miR-938G>A, miR-1302-3C>T and the Risk of Idiopathic Recurrent Pregnancy Loss International Journal of Molecular Sciences, 2021;22(11):. 2021 [PMID: 34200157] (Human)
• C Voukalis, GYH Lip, E Shantsila Effects of antithrombotic drugs on the prothrombotic state in patients with atrial fibrillation: The west Birmingham atrial fibrillation project Thrombosis Research, 2021;200(0):149-155. 2021 [PMID: 33610887] (Human)
• YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages Cancers, 2020;12(12):. 2020 [PMID: 33256011] (Human)
• L Peng, Y Ye, H Mullikin, L Lin, C Kuhn, M Rahmeh, S Mahner, U Jeschke, V von Schönf Expression of trophoblast derived prostaglandin E2 receptor 2 (EP2) is reduced in patients with recurrent miscarriage and EP2 regulates cell proliferation and expression of inflammatory cytokines J Reprod Immunol, 2020;142(0):103210. 2020 [PMID: 33011635] (Human)
• K Zmajkovico, Y Bauer, K Menyhart, M Schnoebele, D Freti, M Boucher, B Renault, R Studer, M Birker-Rob, A Klenk, O Nayler, J Gatfield GPCR-induced YAP activation sensitizes fibroblasts to profibrotic activity of TGFbeta1 PLoS ONE, 2020;15(2):e0228195. 2020 [PMID: 32053631] (Human)
• N Hao, TT Chiou, CH Wu, YY Lei, PL Liang, MC Chao, H Yang, JB Chen Longitudinal Changes of PAI-1, MMP-2, and VEGF in Peritoneal Effluents and Their Associations with Peritoneal Small-Solute Transfer Rate in New Peritoneal Dialysis Patients Biomed Res Int, 2019;2019(0):2152584. 2019 [PMID: 31192253] (Human)
• Y Ye, C Kuhn, M Kösters, GJ Arnold, H Ishikawa-A, C Schulz, N Rogenhofer, CJ Thaler, S Mahner, T Fröhlich, U Jeschke, V von Schönf Anti ?-enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages EBioMedicine, 2019;0(0):. 2019 [PMID: 30827932] (Human)
• JN Justice, AM Nambiar, T Tchkonia, NK LeBrasseur, R Pascual, SK Hashmi, L Prata, MM Masternak, SB Kritchevsk, N Musi, JL Kirkland Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study EBioMedicine, 2019;0(0):. 2019 [PMID: 30616998] (Human)
• Y Ye, A Vattai, N Ditsch, C Kuhn, M Rahmeh, S Mahner, M Ripphahn, R Immler, M Sperandio, U Jeschke, V von Schoen Prostaglandin E2 receptor 3 (EP3) signaling is induced in placentas with unexplained recurrent pregnancy losses (uRPL) Endocr Connect, 2018;0(0):. 2018 [PMID: 29700097] (Human)
• M Jakubowski, J D?bski, E Szahidewic, A Turek-Jaku, J Gawry?, K Gawry?, R Skomro, A Derkacz, A Doroszko Platelet Carbonic Anhydrase II, a Forgotten Enzyme, May Be Responsible for Aspirin Resistance Oxid Med Cell Longev, 2017;2017(0):3132063. 2017 [PMID: 29090039] (Human)
• S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders Sci Rep, 2017;7(1):6871. 2017 [PMID: 28761093] (Human)
• Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies Sci Rep, 2016;6(0):30464. 2016 [PMID: 27456456] (Human)
• Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC. Sci Rep, 0;6(0):29160. 0 [PMID: 27381039] (Human)
• MAP3K19 Is a Novel Regulator of TGF-? Signaling That Impacts Bleomycin-Induced Lung Injury and Pulmonary Fibrosis PLoS ONE, 2016;11(5):e0154874. 2016 [PMID: 27144281] (Human)
• Circadian misalignment increases cardiovascular disease risk factors in humans Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):E1402-11. 2016 [PMID: 26858430] (Human)
• Chang M, Chang H, Chan C, Yeung S, Hsien H, Lin B, Yeh C, Tseng W, Tseng S, Jeng J p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells. PLoS ONE, 2014;9(12):e114446. 2014 [PMID: 25517907] (Human)
• Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients. PLoS ONE, 2014;9(8):e105442. 2014 [PMID: 25133669] (Human)
• Santhekadur P, Akiel M, Emdad L, Gredler R, Srivastava J, Rajasekaran D, Robertson C, Mukhopadhyay N, Fisher P, Sarkar D Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling. FEBS Open Bio, 2014;4(0):353-61. 2014 [PMID: 24918049] (Human)
• Portilla J, Moreno-Perez O, Serna-Candel C, Escoin C, Alfayate R, Reus S, Merino E, Boix V, Giner L, Sanchez-Paya J, Pico A Vitamin D insufficiency and subclinical atherosclerosis in non-diabetic males living with HIV. J Int AIDS Soc, 2014;17(0):18945. 2014 [PMID: 24836607] (Human)
• Hernandez S, Gong J, Chen L, Wu I, Sun J, Keenan H, King G Characterization of circulating and endothelial progenitor cells in patients with extreme-duration type 1 diabetes. Diabetes Care, 2014;37(8):2193-201. 2014 [PMID: 24780357] (Human)
• Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice. PLoS ONE, 2014;9(4):e94373. 2014 [PMID: 24747517] (Human)
• Yamashita A, Zhao Y, Matsuura Y, Yamasaki K, Moriguchi-Goto S, Sugita C, Iwakiri T, Okuyama N, Koshimoto C, Kawai K, Tamaki N, Zhao S, Kuge Y, Asada Y Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage. PLoS ONE, 2014;9(1):e86426. 2014 [PMID: 24466087] (Human)
• Dipyridamole decreases inflammatory metalloproteinase-9 expression and release by human monocytes. Thromb Haemost, 2013;109(2):280-9. 2013 [PMID: 23238437] (Human)
• Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis. BMC Infect Dis, 2012;12(0):144. 2012 [PMID: 22731696] (Human)
• Djafarzadeh R, Sauter M, Notohamiprodjo S, Noessner E, Goyal P, Siess W, Wornle M, Ribeiro A, Himmelein S, Sitter T, Nelson PJ Recombinant GPI-anchored TIMP-1 stimulates growth and migration of peritoneal mesothelial cells. PLoS ONE, 2012;7(4):e33963. 2012 [PMID: 22558080] (Human)
• Lira FS, Pimentel GD, Santos RV, Oyama LM, Damaso AR, Oller do Nascimento CM, Viana VA, Boscolo RA, Grassmann V, Santana MG, Esteves AM, Tufik S, de Mello MT Exercise training improves sleep pattern and metabolic profile in elderly people in a time-dependent manner. Lipids Health Dis, 2011;10(0):113. 2011 [PMID: 21733182] (Human)
• Zhang P, Moudgill N, Hager E, Tarola N, Dimatteo C, McIlhenny S, Tulenko T, DiMuzio PJ Endothelial differentiation of adipose-derived stem cells from elderly patients with cardiovascular disease. Stem Cells Dev., 2011;20(6):977-88. 2011 [PMID: 20879833] (Human)
• Ma Z, Kwong KY, Tovar JP, Paek D Cyclic adenosine monophosphate induces plasminogen activator inhibitor-1 expression in human mast cells. Biochem. Biophys. Res. Commun., 2010;400(4):569-74. 2010 [PMID: 20816667] (Human)
• Patel N, Sundaram N, Yang M Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD). J. Biol. Chem., 2010;285(22):16713-22. 2010 [PMID: 20351105] (Human)
• Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ Human CD4- 8- T cells are a distinctive immunoregulatory subset. FASEB J, 0;24(7):2558-66. 0 [PMID: 20154266] (Human)
• Chang W, Wei K, Jacobs SS, Upadhyay D, Weill D, Rosen GD SPARC suppresses apoptosis of idiopathic pulmonary fibrosis fibroblasts through constitutive activation of beta-catenin. J. Biol. Chem., 2010;285(11):8196-206. 2010 [PMID: 20061390] (Human)
• Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. J. Pathol., 2009;218(1):131-42. 2009 [PMID: 19224541] (Human)
• Tkachuk N, Kiyan J, Tkachuk S, Kiyan R, Shushakova N, Haller H, Dumler I Urokinase induces survival or pro-apoptotic signals in human mesangial cells depending on the apoptotic stimulus. Biochem. J., 2008;415(2):265-73. 2008 [PMID: 18564064] (Human)
• Sharma K, Ramachandrarao S, Qiu G, Usui HK, Zhu Y, Dunn SR, Ouedraogo R, Hough K, McCue P, Chan L, Falkner B, Goldstein BJ Adiponectin regulates albuminuria and podocyte function in mice. J. Clin. Invest., 2008;118(5):1645-56. 2008 [PMID: 18431508] (Human)

Bioinformatics

• Gene Symbol: SERPINE1