Vitronectin Antibody (347312)

• Reactivity: Mu
• Applications: WB
• Clone: 347312
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Vitronectin Antibody (347312) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Vitronectin
  Asp20-Lys478
  Accession # AAH12690
• Specificity: Detects mouse Vitronectin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Vitronectin is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: VTN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vitronectin Antibody (347312)

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alpha _v beta ₃) and thrombin. The 459 aa mature mouse Vitronectin shows 74% amino acid identity with human Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vitronectin Antibody (MAB3875)(3)

Publications using MAB3875

• Sarah J. Parker, Aleksandr Stotland, Elena MacFarlane, Nicole Wilson, Amanda Orosco, Vidya Venkatraman et al. Proteomics reveals Rictor as a noncanonical TGF-beta signaling target during aneurysm progression in Marfan mice American Journal of Physiology-Heart and Circulatory Physiology 2018-11-01 [PMID: 30004239] (Immunohistochemistry, Mouse)
• Sarah J. Parker, Aleksandr Stotland, Elena MacFarlane, Nicole Wilson, Amanda Orosco, Vidya Venkatraman et al. Proteomics reveals Rictor as a noncanonical TGF-beta signaling target during aneurysm progression in Marfan mice American Journal of Physiology-Heart and Circulatory Physiology 2018-11-01 [PMID: 30004239]
• M. Luo, Y. Ji, Y. Luo, R. Li, W. P. Fay, J. Wu Plasminogen activator inhibitor‐1 regulates the vascular expression of vitronectin Journal of Thrombosis and Haemostasis 2017-12-01 [PMID: 29028290]

Bioinformatics

• Gene Symbol: VTN
• Uniprot:
  • AAH12690()