Vitronectin Antibody (1G11E8) - BSA Free

Western Blot: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis using VTN mAb against human VTN (AA: 20-199) recombinant protein. (Expected MW is 45.9 kDa)

Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis of paraffin-embedded liver cancer tissues using VTN mouse mAb with DAB staining.

Flow Cytometry: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis of Hela cells using VTN mouse mAb (green) and negative control (red).

Western Blot: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis using VTN mAb against HEK293 (1) and VTN (AA: 20-199)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Vitronectin Antibody (1G11E8) [NBP2-52586] - Analysis of paraffin-embedded cervical cancer tissues using VTN mouse mAb with DAB staining.

ELISA: Vitronectin Antibody (1G11E8) [NBP2-52586] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 1G11E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Vitronectin Antibody (1G11E8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Vitronectin (AA: 20-199) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VTN
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 54.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Vitronectin Antibody (1G11E8) - BSA Free

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VTN