Vitamin D BP Antibody (JM10-36)

Western Blot: Vitamin D BP Antibody (JM10-36) [NBP2-66786] - Analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Vitamin D BP Antibody (JM10-36) [NBP2-66786] - Staining DBP in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Vitamin D BP Antibody (JM10-36) [NBP2-66786] - Analysis of paraffin-embedded human kidney tissue using anti- DBP antibody. Counter stained with hematoxylin.

Flow Cytometry: Vitamin D BP Antibody (JM10-36) [NBP2-66786] - Analysis of HepG2 cells with DBP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Vitamin D BP Antibody (JM10-36) [NBP2-66786] - Staining DBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Vitamin D BP Antibody (JM10-36) [NBP2-66786] - Staining DBP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Vitamin D BP Antibody (JM10-36) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic pertide within Human Vitamin D BP aa 270-319 / 474. (SwissProt: P02774 Human)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Vitamin D BP Antibody (JM10-36)

• DBP
• DBP/GC
• DBP-MAF
• DBPVDBG
• EC 6.3.1.5
• Gc
• gc-globulin
• Gc-MAF
• GRD3
• group-specific component (vitamin D binding protein)
• Group-specific component
• hDBP
• VDB
• VDBG
• VDBP
• Vitamin D BP
• vitamin D-binding alpha-globulin
• vitamin D-binding protein

Background

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GC