Vitamin D BP Antibody (5E8) - BSA Free

Product Discontinued

Vitamin D BP Antibody (5E8) - BSA Free Summary

• Immunogen: VDCP isolated from human plasma
• Specificity: Clone 5E8 is specific for human Vitamin D Binding Protein (VDBP).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 5E8 reacts strongly with Gc-globulin. In order to measure total Gc-globulin a combination of Clone 05 as capture antibody and Clone 5E8B as biotinylated detection antibody can be used. A strong reaction is also seen in ELISA with Gc-globulin coated directly onto the microtiter well and when tested in sandwich ELISA in combination with a polyclonal antibody against Gc-globulin . In Western blotting after SDS-PAGE, Clone 5E8 reacts with Gc-globulin in both reduced as well as unreduced forms.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Vitamin D BP Antibody (5E8) - BSA Free

• DBP
• DBP/GC
• DBP-MAF
• DBPVDBG
• EC 6.3.1.5
• Gc
• gc-globulin
• Gc-MAF
• GRD3
• group-specific component (vitamin D binding protein)
• Group-specific component
• hDBP
• VDB
• VDBG
• VDBP
• Vitamin D BP
• vitamin D-binding alpha-globulin
• vitamin D-binding protein

Background

Gc-globulin is a plasma protein produced in the liver. Amongst its ligands are vitamin D, thus Gc-globulin is also called vitamin D-binding protein. Gc-globulin is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of Gc-globulin is approximately 50 kDa. The concentration of Gc-globulin in human plasma is app. 400 ug/ml

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GC