Human Versican ELISA Kit (Chemiluminescence)

ELISA: Human Versican ELISA Kit (Chemiluminescence) [NBP2-75353] - Samples were spiked with high concentrations of Human Versican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Versican ELISA Kit (Chemiluminescence) [NBP2-75353] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.50-4000 pg/mL
• Sensitivity: 37.50 pg/mL

Human Versican ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human VS in samples. No significant cross-reactivity or interference between Human VS and analogues was observed.
• Standard Curve Range: 62.50-4000 pg/mL
• Sensitivity: 37.50 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9%
• Intra-Assay: CV% < 7.83%
• Spike Recovery: 89-114%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: VCAN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Versican ELISA Kit (Chemiluminescence)

• Chondroitin sulfate proteoglycan 2WGN
• Chondroitin sulfate proteoglycan core protein 2
• CSPG2
• CSPG2DKFZp686K06110
• ERVR
• GHAP
• Glial hyaluronate-binding protein
• Large fibroblast proteoglycan
• PG-M
• PG-MWGN1
• VCAN
• versican core protein
• versican proteoglycan
• Versican

Background

Versican is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Versican ELISA Kit (NBP2-75353)(1)

Publication using NBP2-75353

• Lima LG, Ham S, Shin H et al. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Nature communications 2021-06-10 [PMID: 34112803] (ELISA)

FAQs for Versican ELISA Kit (NBP2-75353). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VCAN