Recombinant Mouse VEGF120 Protein

SDS-Page: Recombinant Mouse VEGF120 Protein [NBP2-35188]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse VEGF120 Protein Summary

• Description: A disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains with Met at N-terminus corresponding to VEGF120 Source: E. coli

  Uniprot ID: Q00731

  Amino Acid Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

• Details of Functionality: VEGF120 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: VEGFA
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of VEGF120 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 28.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98 % a.a. sequence identity with corresponding regions of rat, 89 % with canine, feline, equine and porcine, and 87 % with human, ovine and bovine VEGF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: VEGFA
• Uniprot:
  • Q00731()
  • Q00731()
  • Q00731()