VEGF-C Antibody (193208) [Unconjugated]

Recombinant Human VEGF‑C protein was serially diluted 2-fold and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (Catalog # MAB752) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 193208
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VEGF-C Antibody (193208) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VEGF-C
  Thr103-Arg227 (Cys156Ser)
  Accession # Q6FH59
• Specificity: Detects human VEGF-C in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPlGF, rhVEGF₁₆₅, rhVEGF₂₀₆, rhVEGF-B₁₈₆, or rhVEGF-D.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VEGFC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF-C Antibody (193208) [Unconjugated]

• Flt4 ligand
• Flt4-L
• vascular endothelial growth factor C
• Vascular endothelial growth factor-related protein
• VEGFC
• VEGF-C
• VRPFLT4 ligand DHM

Background

Vascular Endothelial Growth Factor C (VEGF-C) is a member of the VEGF family of secreted growth factors. The VHD form homodimers after proteolytic removal of the N- and C-terminal pro regions. VEGF-C promotes angiogenesis and lymphangiogenesis through interactions with VEGF R2 and VEGF R3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGF-C Antibody (MAB752)(3)

Publications using MAB752

• Cassuto, J;Folestad, A;Göthlin, J;Malchau, H;Kärrholm, J; VEGF-A, -C, -D, VEGFR1, -2, -3, PDGF-BB and FGF-2 join forces to induce vascular and lymphatic angiogenesis during bone healing of hip implants Bone reports 2025-09-01 [PMID: 40688801] (ELISA Capture, Human)
• Jason Stein, Quangang Xu, Kayla C. Jackson, Elena Romm, Simone C. Wuest, Peter Kosa et al. Intrathecal B Cells in MS Have Significantly Greater Lymphangiogenic Potential Compared to B Cells Derived From Non-MS Subjects Frontiers in Neurology 2018-07-20 [PMID: 30079049]
• Chien MH, Ku CC, Johansson G, Chen MW, Hsiao M, Su JL, Inoue H, Hua KT, Wei LH, Kuo ML Vascular endothelial growth factor-C (VEGF-C) promotes angiogenesis by induction of COX-2 in leukemic cells via the VEGF-R3/JNK/AP-1 pathway. Carcinogenesis, 2009-12-01;30(12):2005-13. 2009-12-01 [PMID: 19825968] (IHC-P, Human)

FAQs for VEGF-C Antibody (MAB752). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
   • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

Bioinformatics

• Gene Symbol: VEGFC
• Uniprot:
  • Q6FH59()