VEGF-B Antibody [Biotin] - 186

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

VEGF-B Antibody [Biotin] - 186 Summary

• Immunogen: E. coli-derived recombinant mouse VEGF-B₁₈₆
• Specificity: Detects mouse VEGF-B₁₈₆ in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) VEGF-B₁₈₆ is observed and less than 1% cross-reactivity with rhVEGF₁₆₅, recombinant mouse (rm) VEGF-B₁₆₇, rhVEGF-C, rmVEGF-D, and rmPlGF-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Vegfa
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF-B Antibody [Biotin] - 186

• vascular endothelial growth factor B
• VEGFB
• VEGF-B
• VEGF-related factor
• VRFVEGFL

Background

Vascular endothelial growth factor B (VEGF-B; also known as VFR) is a member of the VEGF-PDGF supergene family of growth factor molecules (1‑4). Five mouse members have been identified, including VEGF-A, -B, -C, -D, and PlGF(-2) (1, 5). VEGF family members are disulfide-linked homo- and heterodimeric proteins that are important regulators of vasculogenesis and lymphangiogenesis. Two isoforms of mouse VEGF-B are produced by alternative splicing (6, 7). The long form (VEGF₁₈₆) is 207 amino acids (aa) in length, with a putative 21 aa signal sequence and a 186 aa (32 kDa) mature region. The short form (VEGF₁₆₇) is 188 aa in length, with a 21 aa signal sequence and a 167 aa (21 kDa) mature segment. The two isoforms share the same N-terminal 94 aa residue containing the cysteine knot VEGF homology domain (6‑8). VEGF₁₈₆ is O-glycosylated; VEGF₁₆₇ is not. VEGF₁₆₇ binds heparin; VEGF₁₈₆ does not. Thus, VEGF₁₈₆ is secreted and freely diffusible in tissues (7). However, the VEGF-B₁₆₇ isoform is the predominant form in tissue (9). Mouse VEGF-B₁₈₆ shares 93% and 87% aa identity with bovine and human VEGF‑B₁₈₆, respectively. Mouse VEGF-B₁₆₇ also shares 90% and 88% aa identity with bovine and human VEGF-B₁₆₇, respectively. Unlike VEGF₁₆₇, VEGF-B₁₈₆ can undergo proteolytic processing to generate a partially processed 48 kDa heterodimer (16 kDa and 32 kDa) and a fully processed 32 kDa homodimer (two 16 kDa). Processing appears to occur at Arg 127 of the mature protein (10). VEGF-B can heterodimerize with VEGF (7). Both VEGF-B isoforms can bind to VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (11). VEGF-B167 also binds neuropilin-1, but only the 127 aa processed form of VEGF-B₁₈₆ binds neuropilin-1 (10). As a dimer, the full length VEGF-B₁₈₆ does not interact with neuropilin-1, while any dimer that contains the processed VEGF-B₁₂₇ subunit will interact with neuropilin-1 (10). The importance of differential neuropilin binding is unclear. VEGF-B deficient mice display an atrial conduction deficit (12). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1 (11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Vegfa