VEGF 165 Recombinant Protein Summary
A recombinant protein with a His-tag corresponding to amino acids 207 to 371 of Human VEGF 165
Source: E. coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Liquid. In 20mM Tris-HCl(pH8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 21.3kDa (185aa), conformed by MALDI-TOF
Alternate Names for VEGF 165 Recombinant Protein
- vascular endothelial growth factor A
- Vascular permeability factor
- VPFvascular endothelial growth factor
Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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