Recombinant Mouse VEGF 164 Protein

SDS-Page: Mouse VEGF 164 Protein [NBP2-35189]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse VEGF 164 Protein Summary

• Description: A disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains with Met at N-terminus corresponding to VEGF 164 Source: E. coli

  Uniprot ID: Q00731

  Amino Acid Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

• Details of Functionality: VEGF 164 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: VEGFA
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of VEGF 164 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 38.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant mouse VEGF164 contains 165 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of rat, 89 % with human and porcine, 88 % with bovine, and 90 % with feline, equine and canine VEGF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for VEGF 164 Recombinant Protein (NBP2-35189)(1)

Publication using NBP2-35189

• Trinh LT, Osipovich AB, Sampson L et al. Differential regulation of alternate promoter regions in Sox17 during endodermal and vascular endothelial development iScience 2022-09-16 [PMID: 36046192] (BA)
  
  Details:
  20 ng/ml used, mESCs were differentiated to vascular endothelial cells

Bioinformatics

• Gene Symbol: VEGFA
• Uniprot:
  • Q00731()
  • Q00731()
  • Q00731()