Recombinant Human VEGF 121 Protein

• Reactivity: Hu
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Human VEGF 121 Protein Summary

• Description: A disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains corresponding to VEGF 121. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 144-20 kDa in SDS-PAGE under reducing conditions Source: Yeast

  Uniprot ID: P15692-9

  Amino Acid Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is between 0.2-0.4 ng/mL.
• Source: Yeast
• Protein/Peptide Type: Recombinant Protein
• Gene: VEGFA
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of VEGF 121 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, (pH 7.4)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL.

Alternate Names for Recombinant Human VEGF 121 Protein

• MVCD1
• vascular endothelial growth factor A
• Vascular permeability factor
• VEGF-A
• VEGFMGC70609
• VPFvascular endothelial growth factor

Background

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. VEGF121 has increased acidity which allowes it to circulate more freely than other VEGF forms. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: VEGFA
• Uniprot:
  • P15692-9(Human)