VDAC2 Antibody

Western Blot: VDAC2 Antibody [NBP1-68794] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VDAC2 Antibody Summary

• Description: Novus Biologicals Goat VDAC2 Antibody (NBP1-68794) is a polyclonal antibody validated for use in WB and ELISA. Anti-VDAC2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DSAKSKLTRNN corresponding to internal region according to NP_003366.2.
• Epitope: C-DSAKSKLTRNN
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VDAC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 32 kDa band observed in human heart lysates (calculated MW of 31.6 kDa band according to NP_003366.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VDAC2 Antibody

• FLJ23841
• hVDAC2
• Outer mitochondrial membrane protein porin 2
• POR
• VDAC-2
• voltage-dependent anion channel 2
• voltage-dependent anion-selective channel protein 2

Background

VDAC2 forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. VDAC2 interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. It consists mainly of membrane spanning sided beta sheets, and belongs to the eukaryotic mitochondrial porin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDAC2 Antibody (NBP1-68794)(1)

Publication using NBP1-68794

• Chandra D, Choy G, Daniel PT, Tang DG. Bax-dependent regulation of Bak by voltage-dependent anion channel 2. J Biol Chem 2005-05-13 [PMID: 15757910]

Bioinformatics

• Gene Symbol: VDAC2
• Entrez:
  • 7417(Human)
  • 22334(Mouse)
• Uniprot:
  • P45880(Human)