VDAC2 Antibody

Western Blot: VDAC2 Antibody [NB300-871] - Staining (1ug/ml) of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871] - Staining (6ug/ml) of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.

Immunohistochemistry-Paraffin: VDAC2 Antibody [NB300-871] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VDAC2 Antibody Summary

• Description: Novus Biologicals Goat VDAC2 Antibody (NB300-871) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-VDAC2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GHKVGLALELEA corresponding to C-Terminus according to NP_003366.2.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VDAC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 - 6 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx 35 kDa band observed in Human Cerebellum lysates and in preliminary testing of CaCo-2 cell lysate (calculated MW of 31.6kDa according to Human NP_003366.2)
• Theoretical MW: 31.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  VDAC2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VDAC2 Antibody

• FLJ23841
• hVDAC2
• Outer mitochondrial membrane protein porin 2
• POR
• VDAC-2
• voltage-dependent anion channel 2
• voltage-dependent anion-selective channel protein 2

Background

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. Expressed in all tissues examined. Consists mainly of membrane spanning sided beta sheets. Belongs to the eukaryotic mitochondrial porin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDAC2 Antibody (NB300-871)(1)

Publication using NB300-871

• Hoeffer CA, Dey A, Sachan N et al. The Down Syndrome Critical Region Protein RCAN1 Regulates Long-Term Potentiation Memory via Inhibition of Phosphatase Signaling. J Neurosci 2007-11-28 [PMID: 18045910]

Bioinformatics

• Gene Symbol: VDAC2
• Entrez:
  • 7417(Human)
• Uniprot:
  • P45880(Human)