Vav-1 Antibody (9C1)

Western Blot: Vav-1 Antibody (9C1) [H00007409-M01] - VAV1 monoclonal antibody (M01), clone 9C1 Analysis of VAV1 expression in Jurkat.

Immunocytochemistry/Immunofluorescence: Vav-1 Antibody (9C1) [H00007409-M01] - Analysis of monoclonal antibody to VAV1 on HeLa cell. Antibody concentration 10 ug/ml.

Product Discontinued

Vav-1 Antibody (9C1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: VAV1 (AAH13361, 681 a.a. ~ 790 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPAVGSTKYFGTAKARYDFCARDRSELSLKEGDIIKILNKKGQQGWWRGEIYGRVGWFPANYVEEDYSEYC
• Localization: Cytoplasmic
• Specificity: VAV1 - vav 1 oncogene
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAV1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Vav-1 Antibody (9C1)

• oncogene vav
• proto-oncogene vav
• vav 1 guanine nucleotide exchange factor
• vav proto-oncogene
• Vav1
• Vav-1
• vav-T
• VAVvav 1 oncogene

Background

The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAV1
• Entrez:
  • 7409(Human)
• OMIM:
  • 164875(Human)
• Uniprot:
  • AAH13361(Human)