VASP Antibody

Western Blot: VASP Antibody [NBP2-42516] - Staining of Human Platelets lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VASP Antibody Summary

• Description: Novus Biologicals Goat VASP Antibody (NBP2-42516) is a polyclonal antibody validated for use in WB and ELISA. Anti-VASP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKTPKDESANQEEP corresponding to internal region according to NP_003361.1.
• Specificity: This antibody is expected to recognize.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VASP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human platelets lysates is reported in scientific literature (PMID: 1318192).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VASP Antibody

• vasodilator-stimulated phosphoprotein
• VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VASP Antibody (NBP2-42516)(1)

Publication using NBP2-42516

• Wu G, Wei L, Yu A et al. Vasodilator-stimulated phosphoprotein regulates osteosarcoma cell migration. Oncol Rep 2011-12-01 [PMID: 21874265]

Bioinformatics

• Gene Symbol: VASP
• Entrez:
  • 7408(Human)