VASA Overexpression Lysate (Native)

Images

 
Western Blot: VASA Overexpression Lysate (Adult Normal) [NBL1-09796] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VASA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

VASA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210628 and based on accession number NM_024415. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1, mRNA.
Gene
DDX4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
79.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for VASA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VASA Overexpression Lysate (Native)

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91012
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-65214
Species: Hu, Mu, Rt, Ma
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-2437
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB600-749
Species: Hu, Mu, Rt, Rb, Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF6548
Species: Hu, Mu
Applications: IHC
NBP2-37398
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
NB300-232
Species: Mu, Rt, Po, Bv, Ch, Fe, Pa
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00388112-B01P
Species: Hu
Applications: WB, ELISA
AF1997
Species: Hu
Applications: WB, ChIP, ICC
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
AF4090
Species: Hu
Applications: WB, IHC, IP, Neut
NBL1-09796
Species: Hu
Applications: WB

Publications for VASA Lysate (NBL1-09796) (0)

There are no publications for VASA Lysate (NBL1-09796).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VASA Lysate (NBL1-09796) (0)

There are no reviews for VASA Lysate (NBL1-09796). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VASA Lysate (NBL1-09796) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional VASA Products

Bioinformatics Tool for VASA Lysate (NBL1-09796)

Discover related pathways, diseases and genes to VASA Lysate (NBL1-09796). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VASA Lysate (NBL1-09796)

Discover more about diseases related to VASA Lysate (NBL1-09796).
 

Pathways for VASA Lysate (NBL1-09796)

View related products by pathway.

PTMs for VASA Lysate (NBL1-09796)

Learn more about PTMs related to VASA Lysate (NBL1-09796).

Blogs on VASA

There are no specific blogs for VASA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VASA Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DDX4
Entrez