Rat VAP-1/AOC3 ELISA Kit (Colorimetric)

ELISA: Rat VAP-1/AOC3 ELISA Kit (Colorimetric) [NBP2-78770] - Samples were spiked with high concentrations of Rat VAP-1/AOC3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat VAP-1/AOC3 ELISA Kit (Colorimetric) [NBP2-78770] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Rat VAP-1/AOC3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat VAP-1 in samples. No Significant cross-reactivity or interference between Rat VAP-1 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.17%
• Intra-Assay: CV% < 5.46%
• Spike Recovery: 87-103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AOC3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat VAP-1/AOC3 ELISA Kit (Colorimetric)

• amine oxidase, copper containing 3 (vascular adhesion protein 1)
• AOC3
• Copper amine oxidase
• EC 1.4.3
• HPAO
• HPAOSSAO
• Semicarbazide-sensitive amine oxidase
• SSAO
• VAP1
• VAP-1
• VAP1EC 1.4.3.21
• VAP-1membrane primary amine oxidase
• Vascular adhesion protein 1

Background

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been described and is located approximately 9-kb downstream.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for VAP-1/AOC3 ELISA Kit (NBP2-78770)(1)

Publication using NBP2-78770

• Zhang Y, Zhang X, Cao Z Et al. Endothelial cell-derived SSAO can increase MLC20 phosphorylation in VSMCs Open Life Sciences 2021-10-21 [PMID: 34722886] (ELISA)

FAQs for VAP-1/AOC3 ELISA Kit (NBP2-78770). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AOC3