VAMP3/Cellubrevin Antibody (SR3817) - BSA Free

Western Blot: VAMP3/Cellubrevin Antibody (SR3817) [NBP3-27803] - Western blot analysis of A375 cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IP
• Clone: SR3817
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VAMP3/Cellubrevin Antibody (SR3817) - BSA Free Summary

• Description: Novus Biologicals Rabbit VAMP3/Cellubrevin Antibody (SR3817) - BSA Free (NBP3-27803) is a recombinant monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human VAMP3/Cellubrevin (Uniprot #: Q15836)
• Specificity: Detects endogenous levels of total VAMP3/Cellubrevin
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VAMP3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:40
  • Western Blot 1:500-1:2000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for VAMP3/Cellubrevin Antibody (SR3817) - BSA Free

• CEBCellubrevin
• cellubrevin
• SYB3
• Synaptobrevin-3
• VAMP-3
• vesicle-associated membrane protein 3 (cellubrevin)
• vesicle-associated membrane protein 3

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer®s disease, all VAMP isoform levels appear to be significantly reduced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAMP3