VAMP-7 Antibody

Product Discontinued

VAMP-7 Antibody Summary

• Immunogen: Synthetic peptide: C-LKHHSENKGLDKV, representing the internal region of the human protein according to NP_005629.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VAMP7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:128000
• Application Notes: Western Blot: Preliminary experiments gave an approx 48kDa band in Human Lung and Thymus lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.9kDa according to NP_005629.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Human Thymus Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for VAMP-7 Antibody

• FLJ54296
• SYBL1
• SYBL1FLJ53762
• synaptobrevin-like 1
• Synaptobrevin-like protein 1
• tetanus neurotoxin-insensitive VAMP
• Tetanus-insensitive VAMP
• TIVAMP
• TI-VAMP
• VAMP7
• VAMP-7
• VAMP-7FLJ53045
• vesicle-associated membrane protein 7

Background

SYBL1 (Synaptobrevin-like 1) is found ubiquitously and is an unusual v-SNAREs molecule implicated in cellular exocytosis. The SYBL1 gene is found on the pseudoautosomal region (PAR) of X chromosome. It is a highly conserved gene and has been shown to be silenced on inactive X and Y chromosomes. In ICF syndrome, a human disease affecting DNA methylation, SYBL1 escapes from silencing and this correlates with the observed altered patterns of histone methylation and acetylation found. An association between some SYBL1 alleles and bipolar affective disorder (BPAD) has been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAMP7
• Entrez:
  • 6845(Human)
• Uniprot:
  • NP_005629.1(Human)