| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6117-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15595-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80709-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41183-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41183 | |||
| BSA Free | NBP2-41183-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32232 | |||
![Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Western-Blot-NBP1-07118-img0002.jpg "Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of MDCK cells (red). GFP-Tagged human Ti-VAMP (green) and merge (yellow).](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Immunocytochemistry-Immunofluorescence-NBP1-07118-img0001.jpg)
![Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of human spleen.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-158-2-Immunohistochemistry-Paraffin-NBP1-07118-img0003.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) and blotted for VAMP7 (top) and GAPDH (bottom) as loading control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.01855/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Knockdown-Validated-NBP2-32232-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VAMP-7 Antibody [NBP2-32232] - (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) and blotted for VAMP7 (top) and GAPDH (bottom) as loading control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.01855/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Western-Blot-NBP2-32232-img0001.jpg)
![Immunoprecipitation: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for IP at 6 ug per reaction. VAMP7 was also immunoprecipitated by rabbit anti-VAMP7 antibody BL16273. For blotting immunoprecipitated VAMP7, NBP2-32232 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunoprecipitation-NBP2-32232-img0002.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - VAMP7 forms a SNARE complex with SNAP-23 & Syntaxin11. (A) Model illustrating the formation of SNARE complex between VAMP7 (v-SNARE), SNAP-23, & Syntaxin11 (t- SNARE) during cytotoxic granule fusion in human CD8+ T cells. (B) Different Twin-Strep-tag fusion constructs used for pulldown assay. Twin-Strep-tag was fused at the C terminus of VAMP7 & at the N terminus of Syntaxin11 with a (GGS)x3 linker. (C) Western blot of bead stimulated human CD8+ T cells transfected with Twin-Strep-tag-tagged VAMP7, immuno-precipitated with anti-FLAG antibody & detected with antibodies against FLAG, SNAP-23, & Syntaxin11. (D) Western blot of bead stimulated human CD8+ T cells transfected with Twin-Strep-tag-tagged Syntaxin11, immuno-precipitated with anti-Syntaxin antibody & detected with antibodies against Strep-tag, SNAP-23, VAMP7, & VAMP2. As control, cells were transfected with Twin-Strep-tag construct. Ten percentage of the lysates were loaded as input. (E,F) Densitometric quantification of the Western blots shown in (C,D), respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416114749.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - Knockdown of VAMP7 strongly reduces fusion of cytotoxic granules at the IS. (A) Lysates from bead stimulated human CD8+ T cells transfected with either control or VAMP7 siRNAs (1 or 2, respectively) & blotted for VAMP7 (top) & GAPDH (bottom) as loading control. (B) Quantification of VAMP7 protein expression (in % normalized to control siRNA-treated CTLs) performed by densitometry. Bars indicate SEMs. [VAMP7-siRNA1, N = 3; ***p < 0.001 & VAMP7-siRNA2, N = 3; ***p < 0.001 (t-test)]. (C) Human CD8+ T cells co-transfected with granzyme B-mCherry along with either ns-siRNA or VAMP7-siRNA1 or VAMP7-siRNA2 & imaged 12 h after transfection. Selected live-cell TIRF microscopy images of granzyme B-mCherry in a transfected CTL in contact with an anti-CD3 coated coverslip. Fusion events are indicated with open circles (three frames shown per granule fused). (D) Mean percentage of cytotoxic granule fusion in cells transfected with either ns-siRNA (n = 66 & n = 59, respectively) or VAMP7-siRNA1 [n = 91; **p < 0.01 (t-test)] or VAMP7-siRNA2 [n = 72; ***p < 0.001 (t-test)]. (E) Mean average number of granules fused over time in the TIRF plane per cell p = 0.206 (t-test) for VAMP7-siRNA1 & **p < 0.01 (t-test) for VAMP7-siRNA2. Bars indicate mean ± SEM. Scale bar, 5 μm. (F) Calcein-based killing assay for CTLs transfected with either ns-siRNA, VAMP7-siRNA1, or VAMP7-siRNA2. Experiments were carried out in duplicate [N = 4; ***p < 0.001 (t-test)]. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416121985.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-32232] - Fusion of cytotoxic granules with the plasma membrane is insensitive to Tetanus toxin. (A) Bead-stimulated human CD8+ T cells transfected with GFP or TeNT-GFP as indicated. VAMP2 & VAMP7 protein levels were determined by Western blot analysis 12–16 h after transfection. (B) Expression of VAMP2 & VAMP7 protein levels relative to GAPDH in CTLs transfected with GFP or TeNT-GFP as indicated. Graphs represent means [N = 3, ***p < 0.001 (Student's t-test)]. (C) Bead-stimulated human CTLs co-transfected with either GFP or TeNT-GFP along with granzyme B-mCherry & imaged 12 h after transfection. Representative live-cell TIRFM images of CTLs in contact with an anti-CD3 coated coverslip. Fusion events indicated with open arrowheads. (D) Mean percentage of cytotoxic granule fusion in cells transfected with either GFP (n = 50) or TeNT-GFP (n = 40), p = 0.704 (Student's t-test). (E) Mean average number of granules fused per cell over time in the TIRF plane in cells transfected with either GFP (n = 20) or TeNT-GFP (n = 15), p = 0.939 (Student's t-test). Bars show mean ± SEM. Scale bar, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31447853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-32232_rabbit-polyclonal-vamp-7-antibody-310202416114764.jpg)
![Western Blot: VAMP-7 Antibody [NBP3-15595] - analysis of various lysates, using VAMP7 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-15595_rabbit-vamp-7-pab-western-blot-1411202382753..jpg "Western Blot: VAMP-7 Antibody [NBP3-15595] - analysis of various lysates, using VAMP7 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: VAMP-7 Antibody [NBP3-15595] -analysis of Mouse brain, using VAMP7 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/antibody/nbp3-15595_rabbit-vamp-7-pab-western-blot-141120238298..jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP3-15595] - Immunofluorescence analysis of C6 cells using VAMP-7 antibody (NBP3-15595) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15595-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP3-15595] - Immunofluorescence analysis of U2OS cells using VAMP-7 antibody (NBP3-15595) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15595-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-7 Antibody - Azide and BSA Free [VAMP-7] - Immunofluorescence analysis of NIH/3T3 cells using VAMP-7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15595_rabbit-vamp-7-pab-5220251731101.jpg)
![Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP1-80709] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80709-img0006.jpg "Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody [NBP1-80709] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: VAMP-7 Antibody [NBP1-80709] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Immunohistochemistry-Paraffin-NBP1-80709-img0005.jpg)
![Western Blot: VAMP-7 Antibody [NBP2-41183] - Analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/VAMP-7-Antibody-Western-Blot-NBP2-41183-img0003.jpg "Western Blot: VAMP-7 Antibody [NBP2-41183] - Analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: VAMP-7 Antibody - BSA Free [NBP2-41183] - Immunofluorescence of VAMP-7 in human lung tissue with VAMP-7 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41183_rabbit-polyclonal-vamp-7-antibody-2711202420405590.jpg)
![SDS-Page: Recombinant Mouse VAMP-7 His Protein [NBP2-59576] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Mouse-VAMP-7-His-Protein-SDS-Page-NBP2-59576-img0001.jpg "SDS-Page: Recombinant Mouse VAMP-7 His Protein [NBP2-59576] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: VAMP7 Protein [NBP2-22939]](http://images.novusbio.com/fullsize/VAMP7-Protein-SDS-Page-NBP2-22939-img0001.jpg "SDS-Page: VAMP7 Protein [NBP2-22939]")