VAMP-7 Antibody (158.2)

Western Blot: VAMP-7 Antibody (158.2) [NBP1-07118] - Analysis of rat brain extract.

Immunocytochemistry/Immunofluorescence: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of MDCK cells (red). GFP-Tagged human Ti-VAMP (green) and merge (yellow).

Immunohistochemistry-Paraffin: VAMP-7 Antibody (158.2) [NBP1-07118] - Staining of human spleen.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 158.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VAMP-7 Antibody (158.2) Summary

• Immunogen: TI-VAMP recombinant protein
• Specificity: Reacts specifically with the 25 kDa protein of human TI-VAMP protein (human, mouse, rat, canine)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAMP7
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:5-1:200
  • Immunohistochemistry-Paraffin 1:10 - 1:400
  • Western Blot 1:500-1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Cell culture supernatant
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for VAMP-7 Antibody (158.2)

• FLJ54296
• SYBL1
• SYBL1FLJ53762
• synaptobrevin-like 1
• Synaptobrevin-like protein 1
• tetanus neurotoxin-insensitive VAMP
• Tetanus-insensitive VAMP
• TIVAMP
• TI-VAMP
• VAMP7
• VAMP-7
• VAMP-7FLJ53045
• vesicle-associated membrane protein 7

Background

SYBL1 (Synaptobrevin-like 1) involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Found ubiquitously and is an unusual v-SNAREs molecule implicated in cellular exocytosis. The SYBL1 gene is found on the pseudoautosomal region (PAR) of X chromosome. It is a highly conserved gene and has been shown to be silenced on inactive X and Y chromosomes. In ICF syndrome, a human disease affecting DNA methylation, SYBL1 escapes from silencing and this correlates with the observed altered patterns of histone methylation and acetylation found. An association between some SYBL1 alleles and bipolar affective disorder (BPAD) has been observed. SUBUNIT: Component of the SNARE complex composed of STX4, SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAMP-7 Antibody (NBP1-07118)(3)

Publications using NBP1-07118

• Guan Y, Liang X, Ma Z Et al. A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis Nature communications 2021-08-23 [PMID: 34426578] (ICC/IF, Human)
• Saez JJ, Dogniaux S, Shafaq-Zadah M et al. Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism Cells 2021-02-09 [PMID: 33572370] (ICC/IF, Human)
• Spessott Waldo A, Sanmillan Maria L, Kulkarni Vineet V et al. Syntaxin 4 mediates endosome recycling for lytic granule exocytosis in cytotoxic T-lymphocytes. Traffic (Copenhagen, Denmark) 2017-01-01 [PMID: 28471021] (WB, Human)

Bioinformatics

• Gene Symbol: VAMP7
• Entrez:
  • 20955(Mouse)
  • 85491(Rat)