VAMP-5 Antibody (3E5) - Azide and BSA Free

Western Blot: VAMP-5 Antibody (3E5) [NBP3-13515] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-5 antibody [3E5] (NBP3-13515) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

ICC/IF analysis of pLEGFP-C1-hV5p transfected HeLa cells using NBP3-13515 VAMP5 antibody [3E5].Red : Primary antibody

ICC/IF analysis of transfected NRK cells (V5V4-GFP) using NBP3-13515 VAMP5 antibody [3E5].Red : Primary antibody

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 3E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VAMP-5 Antibody (3E5) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: The immunogen used to generate this antibody corresponds to human VAMP-5.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAMP5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for VAMP-5 Antibody (3E5) - Azide and BSA Free

• Myobrevin
• VAMP5
• VAMP-5
• vesicle-associated membrane protein 5 (myobrevin)
• vesicle-associated membrane protein 5

Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAMP5