VAMP-2 Antibody

Western Blot: VAMP-2 Antibody [NBP2-99076] - Anti-VAMP-2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat brain Whole Cell Lysate. Lane B: Mouse brain Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 13 kDa. Observed band size: 17 kDa (We are unsure as to the identity of these extra bands).

Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody [NBP2-99076] - Immunofluorescence staining of VAMP-2 in U87MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human VAMP-2 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP2-99076] - Immunochemical staining of human VAMP-2 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP2-99076] - Immunochemical staining of human VAMP-2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Product Discontinued

VAMP-2 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human VAMP-2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VAMP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for VAMP-2 Antibody

• FLJ11460
• SYB2
• SYB2vesicle-associated membrane protein 2
• synaptobrevin 2
• synaptobrevin-2
• VAMP2
• VAMP-2
• VAMP-2vesicle associated membrane protein 2
• vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAMP2