VAChT/SLC18A3 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VAChT/SLC18A3 Antibody Summary

• Immunogen: A synthetic peptide from amino acid region 450-510 of human VAChT/SLC18A3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
• Localization: Plasma membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC18A3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Western Blot 1:300-1:2000
• Application Notes: IHC-FR reactivity reported in (PMID:24400086).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera with PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for VAChT/SLC18A3 Antibody

• MGC12716
• SLC18A3
• solute carrier family 18 (vesicular acetylcholine), member 3
• Solute carrier family 18 member 3
• solute carrier family 18, member 3
• VAChT
• VACHTvesicular acetylcholine transporter

Background

FUNCTION: Involved in acetylcholine transport into synaptic vesicles.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus. VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle. SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAChT/SLC18A3 Antibody (NB110-74764)(2)

Publications using NB110-74764

• Ezkurdia N, Raurell I, Rodriguez S et al. Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats. PLoS ONE 2014-01-08 [PMID: 24400086] (IHC-Fr, Rat)
• Ezkurdia N, Coll M, Raurell I et al. Blockage of the afferent sensitive pathway prevents sympathetic atrophy and hemodynamic alterations in rat portal hypertension. Liver Int. doi: 10.1111/j.1478-3231.2012.02762.x. 2012-01-31 [PMID: 22292477]

FAQs for VAChT/SLC18A3 Antibody (NB110-74764). (Showing 1 - 2 of 2 FAQs).

1. I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.
   • NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.
2. Would it be possible for you to please let me know the epitopes for NB110-74764?
   • The epitope for NB110-74764 is between aa region 450-505 of human VACHT raised in goat (whole antiserum).

Bioinformatics

• Gene Symbol: SLC18A3
• Entrez:
  • 6572(Human)
  • 20508(Mouse)
  • 60422(Rat)
• Uniprot:
  • Q16572(Human)
  • O35304(Mouse)
  • Q62666(Rat)