UXT Overexpression Lysate (Native)

Images

 
Western Blot: UXT Overexpression Lysate (Adult Normal) [NBL1-17686] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UXT.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

UXT Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200343 and based on accession number NM_004182. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ubiquitously-expressed transcript (UXT), transcript variant 2, mRNA.
Gene
UXT

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for UXT

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UXT Overexpression Lysate (Native)

  • Androgen receptor trapped clone 27 protein
  • ART-27protein UXT
  • SKP2-associated alpha PFD 1
  • STAP1
  • Ubiquitously expressed transcript protein
  • ubiquitously-expressed transcript

Background

This gene encodes a novel protein which is highly conserved in mouse. It interacts with the N-terminus of the androgen receptor and plays a role in facilitating receptor-induced transcriptional activation. It is also likely to be involved in tumorigenesis as it is abundantly expressed in tumor tissues. This gene is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding different isoforms. Transcript Variant: This variant (2) utilizes different 5' end exons compared to variant 1. This results in translation initiation from a downstream Met, and an isoform (2) which is 12 aa shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for UXT Lysate (NBL1-17686) (0)

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Video Protocols

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Bioinformatics

Gene Symbol UXT