UTF1 Antibody [Unconjugated]

UTF1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3958) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). SSEA4 was also detected using Mouse Anti-Human SSEA4 Monoclonal Antibody (Catalog # MAB1435) and stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Cells were counterstained with DAPI (blue). Specific staining of UTF1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

UTF1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human UTF1
  Ser44-Lys153
  Accession # Q5T230
• Specificity: Detects human UTF1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UTF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UTF1 Antibody [Unconjugated]

• undifferentiated embryonic cell transcription factor 1
• UTF1

Background

UTF1 (undifferentiated embryonic cell transcription factor 1) is a 47‑57 kDa transcriptional coactivator. It is expressed mainly in embryonic stem cells, is regulated by Oct-3/4, and promotes rapid cell proliferation. Human UTF1 is a 341 amino acid (aa) phosphoprotein. It is rich in Proline and Arginine residues and contains a
C‑terminal Leu-zipper (aa 286‑313) that is known to interact with the metal-binding motif of ATF-2. Unlike mouse UTF1 which contains an alternate start site at Met43, human UTF1 lacks this Methionine and shows only one isoform. Over amino acids 44‑153, human UTF1 shares 81% aa sequence identity with mouse UTF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UTF1
• Uniprot:
  • Q5T230()