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UTF1 Products By Gene Name

7 Results for "UTF1"

AF7819

UTF1 Antibody

100 μg

AF7819

Western blot shows lysates of D3 mouse embryonic stem cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for UTF1 at approximately 50 and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

D3 mouse embryonic stem cell line untreated (panel A) or treated with 10 mM retinoic acid for 3 days (panel B) was stained with Sheep Anti-Mouse UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7819, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). UTF1 expression is decreased with retinoic acid treatment, as indicated in panel B. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

UTF1 was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). Cells were double-stained using Mouse Anti-Human/Mouse SSEA-1 Monoclonal Antibody (Catalog # MAB2155) and the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of UTF1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7819U-100UG
Alexa Fluor 405	AF7819V-100UG
Alexa Fluor 488	AF7819G-100UG
Alexa Fluor 532	AF7819X-100UG
Alexa Fluor 594	AF7819T-100UG
Alexa Fluor 647	AF7819R-100UG
Alexa Fluor 700	AF7819N-100UG
Alexa Fluor 750	AF7819S-100UG
Allophycocyanin	AF7819A
Biotin	AF7819B
CoraFluor 1	AF7819CL1
DyLight 350	AF7819D
DyLight 405	AF7819E
DyLight 488	AF7819K
DyLight 550	AF7819L
DyLight 594	AF7819M
DyLight 650	AF7819W
DyLight 680	AF7819Y
DyLight 755	AF7819Z
FITC	AF7819F
HRP	AF7819H
Janelia Fluor 525	AF7819JF525
Janelia Fluor 549	AF7819I
Janelia Fluor 646	AF7819J
Janelia Fluor 669	AF7819JF669
PE	AF7819P
PerCP	AF7819C
mFluor Violet 450 SE	AF7819MFV450
mFluor Violet 500 SE	AF7819MFV500
mFluor Violet 610 SE	AF7819MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7819-SP

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AF3958

UTF1 Antibody [Unconjugated]

100 μg

AF3958

UTF1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human UTF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3958) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). SSEA4 was also detected using Mouse Anti-Human SSEA4 Monoclonal Antibody (Catalog # MAB1435) and stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Cells were counterstained with DAPI (blue). Specific staining of UTF1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3958U-100UG
Alexa Fluor 405	AF3958V-100UG
Alexa Fluor 488	AF3958G-100UG
Alexa Fluor 532	AF3958X-100UG
Alexa Fluor 594	AF3958T-100UG
Alexa Fluor 647	AF3958R-100UG
Alexa Fluor 700	AF3958N-100UG
Alexa Fluor 750	AF3958S-100UG
Allophycocyanin	AF3958A
Biotin	AF3958B
CoraFluor 1	AF3958CL1
DyLight 350	AF3958D
DyLight 405	AF3958E
DyLight 488	AF3958K
DyLight 550	AF3958L
DyLight 594	AF3958M
DyLight 650	AF3958W
DyLight 680	AF3958Y
DyLight 755	AF3958Z
FITC	AF3958F
HRP	AF3958H
Janelia Fluor 525	AF3958JF525
Janelia Fluor 549	AF3958I
Janelia Fluor 646	AF3958J
Janelia Fluor 669	AF3958JF669
PE	AF3958P
PerCP	AF3958C
mFluor Violet 450 SE	AF3958MFV450
mFluor Violet 500 SE	AF3958MFV500
mFluor Violet 610 SE	AF3958MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3958-SP

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NBP2-37444

UTF1 Antibody (5B6) - BSA Free

0.1 ml

NBP2-37444

Western Blot: UTF1 Antibody (5B6) [NBP2-37444] - Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-214)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: UTF1 Antibody (5B6) [NBP2-37444] - Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and negative control (purple).

Western Blot: UTF1 Antibody (5B6) [NBP2-37444] - Western blot analysis using UTF1 mAb against human UTF1 recombinant protein. (Expected MW is 32.8 kDa)

ELISA: UTF1 Antibody (5B6) [NBP2-37444] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37444-0.025ml

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NBP3-12788

UTF1 Antibody - Azide and BSA Free

100 ul

NBP3-12788

Western Blot: UTF1 Antibody [NBP3-12788] - Anti-UTF1 rabbit polyclonal antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: UTF1 Antibody [NBP3-12788] - Staining of human UTF1 in human testis with rabbit polyclonal antibody at 1:300 dilution.

Immunoprecipitation: UTF1 Antibody [NBP3-12788] - Lane A:0.5 mg HEK293 Whole Cell LysateLane B:0.5 mg NCI-H292 Whole Cell LysateLane C:0.5 mg K562 Whole Cell Lysate4 uL anti- CLDN6 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti- CLDN6 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 37kDaObserved band size :37 kDa

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-12788-50ul

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NBP1-51943

UTF1 Antibody

(1 Publication)

0.1 mg

NBP1-51943

Western Blot: UTF1 Antibody [NBP1-51943] - Analysis of UTF1 in K562 lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-30992

Alt Catalog# IMG-30992

Conjugate:

Unconjugated

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NBP3-10884

UTF1 Antibody - BSA Free

100 ul

NBP3-10884

Immunohistochemistry: UTF1 Antibody [NBP3-10884] - Immunohistochemical analysis of testis tissue at an antibody concentration of 5ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBL1-17675

UTF1 Overexpression Lysate

0.1 mg

NBL1-17675

Western Blot: UTF1 Overexpression Lysate (Adult Normal) [NBL1-17675] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UTF1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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