USP7 Antibody (JB80-36)

Western Blot: USP7 Antibody (JB80-36) [NBP2-75703] - Analysis of USP7 on different lysates with Rabbit anti-USP7 antibody at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 128 kDa Observed band size: 128kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: USP7 Antibody (JB80-36) [NBP2-75703] - Analysis of paraffin-embedded rat brain tissue using anti-USP7 antibody. Counter stained with hematoxylin.

Flow Cytometry: USP7 Antibody (JB80-36) [NBP2-75703] - Analysis of PC-3M cells with USP7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Western Blot: USP7 Antibody (JB80-36) [NBP2-75703] - Analysis of USP7 on 293T cell using anti-USP7 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: USP7 Antibody (JB80-36) [NBP2-75703] - Analysis of paraffin-embedded human tonsil tissue using anti-USP7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP7 Antibody (JB80-36) [NBP2-75703] - Analysis of paraffin-embedded mouse colon tissue using anti-USP7 antibody. Counter stained with hematoxylin.

Product Discontinued

USP7 Antibody (JB80-36) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human USP7 aa 80-180 / 1,102. (SwissProt: Q93009 Human; SwissProt: Q6A4J8 Mouse; SwissProt: Q4VSI4 Rat)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: USP7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500-1:1000
• Theoretical MW: 128 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for USP7 Antibody (JB80-36)

• Deubiquitinating enzyme 7
• EC 3.1.2.15
• EC 3.4.19.12
• HAUSP
• HAUSPTEF1
• Herpes virus-associated ubiquitin-specific protease
• Herpesvirus-associated ubiquitin-specific protease
• TEF1
• ubiquitin carboxyl-terminal hydrolase 7
• ubiquitin specific peptidase 7 (herpes virus-associated)
• ubiquitin specific protease 7 (herpes virus-associated)
• Ubiquitin thioesterase 7
• Ubiquitin-specific-processing protease 7
• USP7

Background

HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP7